长期给小鼠施用咖啡因对使用卵母细胞进行体外受精和胚胎发育的影响。
Effect of long-term caffeine administration to mice on in vitro fertilization and embryo development using oocytes.
作者信息
Yokota Hidemi, Yokota Yoshimasa, Yokota Mikako, Araki Yasuyuki, Araki Yasuhisa
机构信息
Yokota Maternity Hospital 1-5-22 Shimokoide 371-0031 Maebashi Gunma Japan.
The Institute for Advanced Reproductive Medical Technology 909-21 Ishii, Fujimi 371-0105 Maebashi Gunma Japan.
出版信息
Reprod Med Biol. 2013 May 18;12(4):167-171. doi: 10.1007/s12522-013-0150-4. eCollection 2013 Oct.
PURPOSE
To evaluate the effect of long-term caffeine administration to mice on in vitro fertilization (IVF) of oocytes.
METHODS
Mice were injected with different dosages (0, 0.1, and 1.0 mg/mouse/converted day) of caffeine for one month. Subsequently, the fertilization rate and embryo development to blastocyst stage were evaluated in IVF using oocytes from the mice.
RESULTS
The retrieved average oocyte rate was significantly lower (27.4) in mice injected with 1.0 mg caffeine than in the control group (36.5; < 0.05); the fertilization rate was significantly different between the 0 mg (317/401; 79.1 %) and 1.0 mg group (199/301; 66.1 %) ( < 0.05). At 96 h after insemination, the blastocyst formation rate was significantly decreased in the 1.0 mg group (94/199; 47.2 %) compared with the control (0 mg) group (237/317; 74.8 %) and 0.1 mg group (226/323; 70 %) ( < 0.05). When 1.0 mg caffeine was administered for two weeks, embryo development was significantly impacted.
CONCLUSIONS
Our findings suggest that caffeine administration negatively impacts oocytogenesis and embryonic development after IVF.
目的
评估长期给小鼠注射咖啡因对卵母细胞体外受精（IVF）的影响。
方法
给小鼠注射不同剂量（0、0.1和1.0毫克/只/换算日）的咖啡因，持续一个月。随后，使用这些小鼠的卵母细胞进行体外受精，评估受精率和胚胎发育至囊胚阶段的情况。
结果
注射1.0毫克咖啡因的小鼠，回收的平均卵母细胞率（27.4）显著低于对照组（36.5；P<0.05）；0毫克组（317/401；79.1%）和1.0毫克组（199/301；66.1%）的受精率有显著差异（P<0.05）。授精后96小时，1.0毫克组的囊胚形成率（94/199；47.2%）与对照组（0毫克）（237/317；74.8%）和0.1毫克组（226/323；70%）相比显著降低（P<0.05）。当给予1.0毫克咖啡因两周时，胚胎发育受到显著影响。
结论
我们的研究结果表明，注射咖啡因对体外受精后的卵母细胞发生和胚胎发育有负面影响。
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