Yoshida Mayumi, Hossain M S, Tareq K M A, Obata Ryuichiro, Tsujii Hirotada
Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology Shinshu University 8304 Minamiminowa-mura 399-4598 Nagano Japan.
Reprod Med Biol. 2009 Aug 4;8(4):163-167. doi: 10.1007/s12522-009-0025-x. eCollection 2009 Dec.
Differentiation of endometrial stromal cells into decidual cells occurs during embryo implantation and pregnancy. Recently, it has been reported that relaxin affects the decidualization of cultured human endometrial cells in vitro; however, there has been no study on the decidualization of the Mongolian gerbil (). The authors demonstrated artificially induced decidualization, and the effect of relaxin on decidualization in gerbils.
Ten-to-twelve-week-old female Mongolian gerbils were ovariectomized, treated with estradiol, progesterone, and relaxin, and the uterine horn was stimulated. On day 10, uterine horns were measured for weight, protein concentration, and the incorporation of C-methionine; tissue sections were examined. Interleukin-11 (IL-11) primers were used for RT-PCR to confirm decidualization.
Decidualization can be induced artificially in gerbils. In general, the histological observations of gerbil decidual cells were very similar to those of rats. The uterine horn weight, protein content, and protein synthesis from C-methionine significantly increased in the relaxin-treated gerbils ( 0.05). Mast cells in the relaxin-treated uterus had proliferated more than those of the non-relaxin-treated group, which was confirmed by IL-11 expression.
We conclude that decidualization can be induced artificially, and relaxin increased weight of uterine horn, protein concentration, protein synthesis and IL-11 expression in gerbils.
子宫内膜基质细胞向蜕膜细胞的分化发生在胚胎着床和妊娠期间。最近，有报道称松弛素在体外影响培养的人子宫内膜细胞的蜕膜化；然而，尚未有关于长爪沙鼠蜕膜化的研究。作者证明了长爪沙鼠人工诱导的蜕膜化以及松弛素对其蜕膜化的影响。
对10至12周龄的雌性长爪沙鼠进行卵巢切除，用雌二醇、孕酮和松弛素处理，并刺激子宫角。在第10天，测量子宫角的重量、蛋白质浓度以及C-蛋氨酸的掺入量；检查组织切片。使用白细胞介素-11（IL-11）引物进行逆转录聚合酶链反应（RT-PCR）以确认蜕膜化。
长爪沙鼠可人工诱导蜕膜化。一般来说，长爪沙鼠蜕膜细胞的组织学观察与大鼠非常相似。在松弛素处理的长爪沙鼠中，子宫角重量、蛋白质含量以及C-蛋氨酸的蛋白质合成显著增加（P<0.05）。通过IL-11表达证实，松弛素处理的子宫中的肥大细胞比未用松弛素处理的组增殖更多。
我们得出结论，长爪沙鼠可人工诱导蜕膜化，并且松弛素增加了长爪沙鼠子宫角的重量、蛋白质浓度、蛋白质合成和IL-11表达。
