Export Inspection Council, 3rd Floor - NDYMCA Cultural Centre Building, 1, Jai Singh Road, New Delhi 110 001, India.
Export Inspection Agency-Kolkata (Laboratory), 101, Southend Conclave, 1582, Rajdanga Main Road, Kolkata 700 107, India.
Food Chem. 2018 Sep 15;260:336-343. doi: 10.1016/j.foodchem.2018.04.018. Epub 2018 Apr 9.
An accurate, reliable and fast multi-residue, multi-class method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed and validated for simultaneous determination and quantification of 24 pharmacologically active substances of three different classes (Quinolones including fluoroquinolones, sulphonamides and tetracyclines) in aquaculture shrimps. Sample preparation involves extraction with acetonitrile containing 0.1% formic acid and followed by clean up with n-hexane and 0.1% methanol in water by UPLC-MS/MS within 8 min. The method was validated according to European Commission Decision 2002/657. Acceptable values were obtained for linearity (5-200 μg kg), specificity, Limit of Quantification (5-10 μg kg), recovery (between 83 and 100%), repeatability (RSD < 9%), within lab reproducibility (RSD < 15%), reproducibility (RSD ≤ 22%), decision limit (105-116 μg kg) and detection capability (110-132 μg kg). The validated method was applied to aquaculture shrimp samples from India.
建立并验证了一种超高效液相色谱-串联质谱（UPLC-MS/MS）法，用于同时检测和定量养殖虾中三类 24 种具有不同药理活性的物质（包括氟喹诺酮类、磺胺类和四环素类的喹诺酮类）。样品制备包括在含 0.1%甲酸的乙腈中提取，然后用 UPLC-MS/MS 用正己烷和 0.1%甲醇/水在 8 min 内进行净化。该方法根据欧洲委员会第 2002/657 号决定进行了验证。线性（5-200 μg/kg）、特异性、定量限（5-10 μg/kg）、回收率（83-100%）、重复性（RSD<9%）、实验室内再现性（RSD<15%）、再现性（RSD≤22%）、决策限（105-116 μg/kg）和检测能力（110-132 μg/kg）均获得可接受值。该方法已应用于来自印度的养殖虾样品。
