Silva E, Martins C, Pereira A S, Loureiro S, Cerejeira M J
LEAF, Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017, Lisboa, Portugal.
Department of Biology and CESAM, University of Aveiro, 3810-193, Aveiro, Portugal.
Ecotoxicology. 2018 Sep;27(7):956-967. doi: 10.1007/s10646-018-1938-0. Epub 2018 Apr 26.
In a regulatory perspective addressing the cumulative effect of co-occurring chemicals is the first and most important step in providing a more realistic hazard assessment of chemical cocktails to both man and environment. This study was conducted to show if joint effects on the immobilisation of the crustacean Daphnia magna and on the growth inhibition of algae Raphidocelis subcapitata follow additivity (concentration addition (CA) or independent action (IA) predictions) or if there is an interaction between chemicals in the organisms upon exposure to an environmentally realistic mixture of chlorpyrifos and terbuthylazine, with expected different molecular sites of action. A pattern of antagonism at lower doses and synergism at higher doses was found for acute immobility data, while no deviation from the additive conceptual models was observed in the algae inhibition test. Results in relation to the relevant set of regulatory acceptable concentrations (RACs) and environmental quality standards (EQSs) derived for individual chlorpyrifos and terbuthylazine were evaluated.
从监管角度来看，解决同时存在的化学物质的累积效应是对化学混合物对人类和环境进行更现实的危害评估的首要也是最重要的一步。本研究旨在表明，对于甲壳类大型溞的固定化以及藻类斜生栅藻的生长抑制的联合效应是否遵循相加性（浓度相加（CA）或独立作用（IA）预测），或者在接触环境现实中的毒死蜱和特丁津混合物时，生物体中的化学物质之间是否存在相互作用，这两种化学物质预期具有不同的分子作用位点。对于急性固定化数据，在较低剂量下发现了拮抗模式，在较高剂量下发现了协同模式，而在藻类抑制试验中未观察到与相加概念模型的偏差。评估了与针对单个毒死蜱和特丁津得出的相关监管可接受浓度（RACs）和环境质量标准（EQSs）相关的结果。
