Zhu Li, Aly Mostafa, Wang Haihao, Karakizlis Hristos, Weimer Rolf, Morath Christian, Kuon Ruben Jeremias, Toth Bettina, Ekpoom Naruemol, Opelz Gerhard, Daniel Volker
Transplantation-Immunology, Institute of Immunology, University Hospital Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany; Department of Hematology, Tongji Hospital, Huazhong University of Science and Technology, 430030 Wuhan, China.
Transplantation-Immunology, Institute of Immunology, University Hospital Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany; Nephrology Unit, Internal Medicine Department, Assiut University, Egypt.
Transpl Immunol. 2018 Aug;49:59-71. doi: 10.1016/j.trim.2018.04.005. Epub 2018 Apr 24.
There is evidence that NK cells with low cytotoxicity but strong immunoregulatory characteristics contribute to good graft outcome. We attempted to investigate which NK cell subsets increase post-transplant and might affect graft function.
Lymphocyte and NK cell subsets were determined in whole blood using eight-colour-fluorescence flow cytometry in patients pre-transplant and post-transplant. In total, 31 transplant recipients were studied.
When cell numbers were compared in 9 patients pre- and 6 months post-transplant, post-transplant CD56dimCD16+ (p = 0.011) NK cells with the phenotype CD158a+ (p = 0.008), CD158e+ (p = 0.038), NKG2A+ (p = 0.008), NKG2D+ (p = 0.011), IFNyR+ (p = 0.008), perforin+ (p = 0.008), granzymeB+ (p = 0.008), perforin+granzymeB+ (p = 0.008) and perforin-granzymeB- (p = 0.021) were lower than those pre-transplant, indicating a post-transplant reduction of cytotoxic NK cells. In 28 patients NK cell subsets were analyzed with respect to time post-transplant (median 888 days post-transplant). CD56dimCD16+ NK cells co-expressing CD158a (p = 0.014), NKG2D (p = 0.047), IL4R (p = 0.038), IL10R (p = 0.008) and IFNy (p = 0.036) as well as CD56bright NK cells with the phenotype TGFß+ (p = 0.017), TGFR+ (p = 0.035), CD158a+ (p = 0.042) and perforin-granzymeB- (p = 0.048) increased with time post-transplant.
Post-transplant, cytotoxic NK cells were lower than pre-transplant and remained low, whereas NK cell subsets with potentially immunoregulatory properties increased.
有证据表明，细胞毒性低但具有强大免疫调节特性的自然杀伤（NK）细胞有助于移植获得良好结果。我们试图研究移植后哪些NK细胞亚群会增加以及可能影响移植功能。
采用八色荧光流式细胞术对31例移植受者移植前和移植后的全血中的淋巴细胞和NK细胞亚群进行检测。
在比较9例患者移植前和移植后6个月的细胞数量时，移植后具有CD158a +（p = 0.008）、CD158e +（p = 0.038）、NKG2A +（p = 0.008）、NKG2D +（p = 0.011）、IFNyR +（p = 0.008）、穿孔素+（p = 0.008）、颗粒酶B +（p = 0.008）、穿孔素+颗粒酶B +（p = 0.008）和穿孔素 - 颗粒酶B -（p = 0.021）表型的CD56dimCD16 +（p = 0.011）NK细胞低于移植前，表明移植后细胞毒性NK细胞减少。在28例患者中，分析了移植后时间（移植后中位数888天）的NK细胞亚群。共表达CD158a（p = 0.014）、NKG2D（p = 0.047）、IL4R（p = 0.038）、IL10R（p = 0.008）和IFNy（p = 0.036）的CD56dimCD16 + NK细胞以及具有TGFß +（p = 0.017）、TGFR +（p = 0.035）、CD158a +（p = 0.042）和穿孔素 - 颗粒酶B -（p = 0.048）表型的CD56bright NK细胞随移植后时间增加。
移植后，细胞毒性NK细胞低于移植前且维持在低水平，而具有潜在免疫调节特性的NK细胞亚群增加。
