Laboratory for Agricultural Wastes Treatment and Recycling, Circular Agriculture Research Center, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Xuanwu District, Nanjing, 210014, Jiangsu Province, China.
Nanjing Institute of Agricultural Sciences in Jiangsu Hilly Area, No.6 Xianyin South Road, Qixia District, Nanjing, 210046, Jiangsu Province, China.
Appl Microbiol Biotechnol. 2018 Jul;102(13):5717-5729. doi: 10.1007/s00253-018-9013-6. Epub 2018 Apr 27.
Soil weakness across consecutive cropping fields can be partially explained by the changes in microbial community diversity and structure. Succession patterns and co-occurrence mechanisms of bacteria and fungi, especially beneficial or pathogenic memberships in continuous cropping strawberry fields and their response to edaphic factors remained unclear. In this study, Illumina sequencing of bacterial 16S ribosomal RNA and fungal internal transcribed spacer genes was applied in three time-course (1, 5, and 10 years) fields across spring and winter. Results showed that the richness and diversity of bacterial and fungal communities increased significantly (p < 0.05) in 1-year field and decreased afterwards across two seasons. Network analysis revealed beneficial bacterial and fungal genus (Bacillus and Trichoderma) dominated under 1-year field whereas Fusarium accumulated under 10-year field at either season. Moreover, Trichoderma harzianum and Bacillus subtilis that have been reported to effectively control Fusarium wilt in strawberries accumulated significantly under 1-year field. Canonical correspondence analysis showed that beneficial bacterial Rhodospirillales and Rhizobiales and fungal Glomerales accumulated in 1-year field and their distributions were significantly affected by soil pH, microbial biomass C (MBC), and moisture. On the contrary, fungal pathogenic species Fusarium oxysporum strongly increased under 10-year field at the winter sample and the abundance was positively (p < 0.01) correlated with soil moisture. Our study suggested that the potential of microcosm under 1-year field stimulates the whole microbial diversity and favors different beneficial taxa across two seasons. Soil pH, moisture, and MBC were the most important edaphic factors leading to contrasting beneficial and pathogenic memberships across consecutive strawberry cropping fields.
连续种植田土壤肥力的下降可以部分解释为微生物群落多样性和结构的变化。细菌和真菌的演替模式和共同发生机制，特别是连续种植草莓田中有益或致病的成员及其对土壤因素的响应仍然不清楚。在这项研究中，应用 Illumina 测序对细菌 16S 核糖体 RNA 和真菌内部转录间隔区基因进行了三个时间序列（1、5 和 10 年）的春季和冬季田间研究。结果表明，细菌和真菌群落的丰富度和多样性在 1 年生田间显著增加（p<0.05），随后在两个季节中下降。网络分析显示，有益的细菌属（芽孢杆菌属和木霉属）在 1 年生田占主导地位，而镰刀菌属在 10 年生田在两个季节中积累。此外，已报道能有效防治草莓枯萎病的木霉属和芽孢杆菌属在 1 年生田积累量显著增加。典范对应分析表明，有益的细菌 Rhodospirillales 和 Rhizobiales 和真菌 Glomerales 在 1 年生田积累，其分布受到土壤 pH、微生物生物量 C（MBC）和水分的显著影响。相反，真菌病原种尖孢镰刀菌在冬季样本中在 10 年生田大量增加，其丰度与土壤水分呈正相关（p<0.01）。我们的研究表明，1 年生田的微宇宙潜力刺激了整个微生物多样性，并有利于两个季节的不同有益类群。土壤 pH、水分和 MBC 是导致连续草莓种植田有益和致病成员形成对比的最重要土壤因素。
