Shen Qinke, Wang Yuxian, Shen Jian, Jiang Ling, Wei Ce, Zhang Hongman
College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, China.
College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China.
Curr Microbiol. 2018 Sep;75(9):1133-1141. doi: 10.1007/s00284-018-1499-8. Epub 2018 Apr 27.
Fatty acids (FAs) are one of the important factors that can influence cell growth and membrane composition. The aim of this study was to investigate the influence of supplementing MLM growth medium with C18 free fatty acids (C-FFAs), including stearic (C18:0), oleic (C18:1), linoleic (C18:2), and linolenic (C18:3) acid, on the growth of Lactobacillus plantarum CICC21001 by forming ion pairs with lysine to increase the solubility of FAs in liquid medium. The utilization of C-FFAs was further confirmed by GC-FID. The investigation of cell properties, including cell surface hydrophobicity and zeta potential, was carried out for the modified L. plantarum and control group (non-supplementation). Furthermore, cell survival was measured in real time under heat (at 55 and 62 °C for 5 min), acid (pH 2.2), and bile salt stress. Our results indicated that the action of L. plantarum was modulated by assimilating C-FFAs. This study suggested that C-FFAs altered the life cycles and physiochemical properties of L. plantarum, which provided a guideline for probiotics production and their medical application.
脂肪酸（FAs）是影响细胞生长和膜组成的重要因素之一。本研究的目的是通过与赖氨酸形成离子对来提高脂肪酸在液体培养基中的溶解度，从而研究在MLM生长培养基中添加C18游离脂肪酸（C-FFAs），包括硬脂酸（C18:0）、油酸（C18:1）、亚油酸（C18:2）和亚麻酸（C18:3），对植物乳杆菌CICC21001生长的影响。通过气相色谱-火焰离子化检测（GC-FID）进一步证实了C-FFAs的利用情况。对改良后的植物乳杆菌和对照组（未添加）进行了细胞特性研究，包括细胞表面疏水性和zeta电位。此外，还实时测定了在热（55和62℃处理5分钟）、酸（pH 2.2）和胆盐胁迫下的细胞存活率。我们的结果表明，植物乳杆菌的作用通过同化C-FFAs得到调节。本研究表明，C-FFAs改变了植物乳杆菌的生命周期和理化性质，为益生菌的生产及其医学应用提供了指导。
