College of Resources and Environment, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer, Key Laboratory of Colleges and Universities in Shandong Province Agricultural Environment, Shandong Agricultural University, Tai'an, 271018, China.
Shandong Research Institute of Coal Geological Planning and Prospecting, Shandong Zhengdao Resources and Environment Developing Co., Ltd., Jinan, 250101, China.
Environ Sci Pollut Res Int. 2018 Jul;25(19):18570-18578. doi: 10.1007/s11356-018-2031-1. Epub 2018 Apr 27.
Copper (Cu) is one of the most commonly detected heavy metals in livestock manure pollution. Protaetia brevitarsis Lewis larvae are widely used in the decomposition of livestock manure. During decomposition, heavy metals in livestock manure can accumulate in P. brevitarsis larvae and affect normal growth and reproduction. Therefore, this research focused on characterizing the toxic effects of Cu to P. brevitarsis Lewis larvae. Larvae were exposed to Cu concentrations of 0, 100, 200, 400, and 800 mg kg in edible fungi residue for 7, 14, 21, and 28 days. Results showed that the soluble protein content was markedly increased in 200 and 400 mg kg treatment groups on day 28. Compared to the control group, superoxide dismutase (SOD) and glutathione S-transferase (GST) activities were significantly stimulated on day 7 and then decreased as exposure length was increased (e.g., after 14, 21, and 28 days). Catalase (CAT) activity was also significantly increased after 7 days of exposure. Malondialdehyde (MDA) levels were markedly increased in the 100, 200, and 400 mg kg treatment groups on day 7. However, as time and Cu concentration were increased, MDA levels gradually decreased. These results indicate that Cu has biochemical effects on P. brevitarsis Lewis larvae, and both time and dose affect this biochemical response.
铜（Cu）是牲畜粪便污染中最常见的重金属之一。暗黑鳃金龟幼虫被广泛应用于牲畜粪便的分解。在分解过程中，牲畜粪便中的重金属会在暗黑鳃金龟幼虫体内积累，并影响其正常生长和繁殖。因此，本研究重点研究了 Cu 对暗黑鳃金龟幼虫的毒性作用。幼虫在食用菌残渣中暴露于 0、100、200、400 和 800mg/kg 的 Cu 浓度下 7、14、21 和 28 天。结果表明，在第 28 天，200 和 400mg/kg 处理组的可溶性蛋白含量明显增加。与对照组相比，超氧化物歧化酶（SOD）和谷胱甘肽 S-转移酶（GST）活性在第 7 天被显著刺激，然后随着暴露时间的延长而降低（例如，在 14、21 和 28 天后）。过氧化氢酶（CAT）活性在暴露 7 天后也显著增加。丙二醛（MDA）水平在第 7 天的 100、200 和 400mg/kg 处理组中明显增加。然而，随着时间和 Cu 浓度的增加，MDA 水平逐渐降低。这些结果表明，Cu 对暗黑鳃金龟幼虫具有生化效应，并且时间和剂量都影响这种生化反应。
