Nolasco Matheus, Biondi Ilka, Pimenta Daniel C, Branco Alexsandro
Laboratory of Venomous Animals and Herpetology, Biology Department, State University of Feira de Santana - UEFS, Feira de Santana, BA, 44036-900, Brazil.
Laboratory of Biochemistry and Biophysics, Instituto Butantan, São Paulo, SP, 05503-900, Brazil.
Toxicon. 2018 Aug;150:74-76. doi: 10.1016/j.toxicon.2018.04.023. Epub 2018 Apr 26.
Arthropod venoms may be considered important sources of bioactive molecules; however, technical difficulties, such as venom extraction and homogeneity may impair the biochemical identification of new molecules. In this context, we have developed a method to maintain wasps in captivity that allows the collection of the venom, without use of chemical, mechanical or electrical stimuli. The crude venom was analyzed by RP-HPLC-ESIQ-ToF and 20 peptides were identified by de novo peptide sequencing, among them Eumenine-Mastoparan and a Ponericin-G2-simile peptide.
节肢动物毒液可能被认为是生物活性分子的重要来源；然而，诸如毒液提取和均一性等技术难题可能会妨碍新分子的生化鉴定。在此背景下，我们开发了一种圈养黄蜂的方法，该方法无需使用化学、机械或电刺激就能收集毒液。通过反相高效液相色谱-电喷雾离子化-飞行时间质谱（RP-HPLC-ESIQ-ToF）对粗毒液进行分析，并通过从头肽测序鉴定出20种肽，其中包括胡蜂肽-马斯托帕兰和一种类庞尼菌素-G2肽。
