Sosnicki A, Cassens R G
Muscle Biology Laboratory, University of Wisconsin, Madison 53706.
Poult Sci. 1988 Jun;67(6):973-8. doi: 10.3382/ps.0670973.
Muscle fiber subtypes, determined with the actomyosin Ca+2,Mg+2-adenosine triphosphatase (ATPase) reaction in chicken anterior latissimus dorsi and posterior latissimus dorsi muscles, were demonstrated only after acid or alkaline preincubation followed by a 60-min enzyme incubation. In contrast, subtypes were demonstrated in the sartorius muscle either with or without preincubation. A single-step procedure was therefore possible with this muscle. The results were generally similar to those obtained previously with the mycosin Ca+2-ATPase procedure. Both methods revealed corresponding muscle fiber subtypes, with the exceptions noted below. The actomyosin Ca+2,Mg+2-ATPase procedure, following preincubation at pH 9.4 and 10.3, resulted in a similar reaction intensity in all fiber types. With the myosin Ca+2-ATPase procedure, the IRA (slow) type in anterior latissimus dorsi and sartorius muscles and the I (slow), IIR (fast oxidative-glycolytic), and IIW (fast glycolytic) types in posterior latissimus dorsi muscle had a higher reaction intensity following preincubation at pH 9.4 than at pH 10.3. Fiber Types IIR and IIW in sartorius muscle were easily distinguished with the actomyosin Ca+2,Mg+2-ATPase procedure.
通过肌动球蛋白Ca+2、Mg+2 - 三磷酸腺苷酶（ATPase）反应确定的鸡背阔肌前肌和背阔肌后肌中的肌纤维亚型，仅在酸或碱预孵育后再进行60分钟酶孵育后才能显示出来。相比之下，缝匠肌中的亚型无论有无预孵育均可显示。因此，对于这块肌肉可以采用单步程序。结果总体上与先前用肌球蛋白Ca+2 - ATPase程序获得的结果相似。两种方法都揭示了相应的肌纤维亚型，但有以下例外情况。在pH 9.4和10.3预孵育后，肌动球蛋白Ca+2、Mg+2 - ATPase程序在所有纤维类型中产生相似的反应强度。对于肌球蛋白Ca+2 - ATPase程序，背阔肌前肌和缝匠肌中的IRA（慢）型以及背阔肌后肌中的I（慢）、IIR（快氧化 - 糖酵解）和IIW（快糖酵解）型在pH 9.4预孵育后的反应强度高于pH 10.3预孵育后的反应强度。缝匠肌中的IIR型和IIW型纤维通过肌动球蛋白Ca+2、Mg+2 - ATPase程序很容易区分。
