Pan Z Q, Prives C
Department of Biological Sciences, Columbia University, New York, NY 10027.
Science. 1988 Sep 9;241(4871):1328-31. doi: 10.1126/science.2970672.
Oligonucleotides complementary to regions of U1 and U2 small nuclear RNAs (snRNAs), when injected into Xenopus laevis oocytes, rapidly induced the specific degradation of U1 and U2 snRNAs, respectively, and then themselves were degraded. After such treatment, splicing of simian virus 40 (SV40) late pre-mRNA transcribed from microinjected viral DNA was blocked in oocytes. If before introduction of SV40 DNA into oocytes HeLa cell U1 or U2 snRNAs were injected and allowed to assemble into small nuclear ribonucleoprotein particle (snRNP)-like complexes, SV40 late RNA was as efficiently spliced as in oocytes that did not receive U1 or U2 oligonucleotides. This demonstrates that oocytes can form fully functional hybrid U1 and U2 snRNPs consisting of human snRNA and amphibian proteins.
与U1和U2小核RNA（snRNA）区域互补的寡核苷酸，当注射到非洲爪蟾卵母细胞中时，分别迅速诱导U1和U2 snRNA的特异性降解，然后自身也被降解。经过这样的处理后，从显微注射的病毒DNA转录而来的猴病毒40（SV40）晚期前体mRNA的剪接在卵母细胞中被阻断。如果在将SV40 DNA引入卵母细胞之前，注射HeLa细胞的U1或U2 snRNA并使其组装成小核核糖核蛋白颗粒（snRNP）样复合物，SV40晚期RNA的剪接效率与未接受U1或U2寡核苷酸的卵母细胞相同。这表明卵母细胞可以形成由人snRNA和两栖类蛋白质组成的功能完全正常的杂交U1和U2 snRNP。
