Sarasija Shaarika, Norman Kenneth R
Department of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, NY, USA.
Bio Protoc. 2018 Apr 5;8(7). doi: 10.21769/BioProtoc.2801.
Mitochondrial function is altered in various pathologies, highlighting the crucial role mitochondria plays in maintaining cellular homeostasis. Mitochondrial structure undergoes constant fission and fusion in response to changing cellular environment. Due to this, analyzing mitochondrial structure could provide insight into the physiological state of the cell. In this protocol, we describe a method to analyze mitochondrial structure in body wall muscles in the nematode using both transgenic and dye-based approaches.
线粒体功能在各种病理状态下都会发生改变，这突出了线粒体在维持细胞稳态中所起的关键作用。线粒体结构会根据细胞环境的变化不断进行裂变和融合。因此，分析线粒体结构可以深入了解细胞的生理状态。在本实验方案中，我们描述了一种使用转基因和基于染料的方法来分析线虫体壁肌肉中线粒体结构的方法。
