Hampton J A, Lantz R C, Goldblatt P J, Lauren D J, Hinton D E
Department of Anatomy, School of Medicine, West Virginia University, Morgantown 26506.
Anat Rec. 1988 Jun;221(2):619-34. doi: 10.1002/ar.1092210208.
The intrahepatic biliary system was studied in the rainbow trout (Salmo gairdneri), a teleost known to form liver neoplasms after exposure to various carcinogens. Normal adults (N = 25) were examined using light microscopic, enzyme histochemical, and transmission and scanning electron microscopic methods. In light micrographs, longitudinal arrays of hepatocytes appeared as double rows incompletely divided by elongated darkly stained cells. Electron micrographs showed tubules of five to nine pyramidally shaped hepatocytes with their apices directed toward a central biliary passageway and their bases directed toward sinusoids. Sequentially, beginning with hepatocytes, biliary passageways included canaliculi, preductules, ductules, and ducts. Canaliculi were short and joined transitional passageways (preductules) formed by junctional complexes between plasma membranes of hepatocytes and small, electron-dense cells with a high nuclear to cytoplasmic ratio. Ductules, completely lined by biliary epithelial cells, occupied central regions of hepatic tubules. Relatively elongated, ductular cells were intimately associated with surrounding hepatocytes, separated from them by only a thin extracellular space devoid of a basal lamina. Epithelium of bile ducts included cuboidal through mucus-laden columnar cells, surrounded by basal lamina and, in larger ducts, by fibroblasts, smooth muscle cells, and a capillary plexus. Bile ducts and hepatic arterioles, but not venules, were distributed together. The ultrastructure of biliary epithelium, periductular, and periductal cells is presented.
对虹鳟鱼（Salmo gairdneri）的肝内胆管系统进行了研究，虹鳟鱼是一种硬骨鱼，已知在接触各种致癌物后会形成肝脏肿瘤。使用光学显微镜、酶组织化学、透射电子显微镜和扫描电子显微镜方法对25只正常成年虹鳟鱼进行了检查。在光学显微镜照片中，肝细胞的纵向排列呈现为被细长的深色细胞不完全分隔的双排结构。电子显微镜照片显示，由五到九个金字塔形肝细胞组成的小管，其顶端指向中央胆管通道，基部指向血窦。依次地，从肝细胞开始，胆管通道包括胆小管、前小胆管、小胆管和胆管。胆小管很短，连接着由肝细胞质膜与核质比高的小的电子致密细胞之间的连接复合体形成的过渡通道（前小胆管）。小胆管完全由胆管上皮细胞衬里，占据肝小管的中央区域。相对细长的小胆管细胞与周围的肝细胞紧密相连，仅被一个没有基膜的薄细胞外空间分隔。胆管上皮包括立方状至充满黏液的柱状细胞，被基膜包围，在较大的胆管中，还被成纤维细胞、平滑肌细胞和毛细血管丛包围。胆管和肝小动脉（而非小静脉）分布在一起。本文展示了胆管上皮、小胆管周围和胆管周围细胞的超微结构。
