Hampton J A, Lantz R C, Hinton D E
Department of Anatomy, School of Medicine, West Virginia University, Morgantown 26506.
Am J Anat. 1989 May;185(1):58-73. doi: 10.1002/aja.1001850107.
Hepatic stroma and parenchyma with its component cell types were quantitatively described in adult male and female actively-spawning 5-year-old rainbow trout (Salmo gairdneri, Richardson). Point-count morphometry of glycol methacrylate sections estimated volume compartments for stroma and parenchyma. Veins composed 85% of the stroma while arteries and bile ducts occupied approximately 6-7% each. Parenchyma accounted for 95% of hepatic volume. Point-count morphometry of transmission electron micrographs estimated volume compartments as well as numerical and surface density measurements for parenchymal components. Within the hepatic parenchymal compartment, hepatocytes occupied 85% and showed significant sex differences. Female hepatocytes were significantly more numerous but were smaller, only 60% of the volume of male hepatocytes. Since hepatocyte nuclear volume was equal in both sexes, differences were due to reduced cytoplasmic volume in females. Perisinusoidal macrophages of females occupied larger volumes of their respective parenchymal compartments, and their larger mean cytoplasmic volumes suggested activation. Biliary epithelial cells of preductules and ductules were numerous. Ratios of numerical density of hepatocytes to biliary epithelial cells were consistent with a tubular arrangement of hepatocytes. Factors possibly mediating the sexual dimorphism are discussed.
对成年雄性和雌性正在积极产卵的5岁虹鳟鱼（Salmo gairdneri，Richardson）的肝基质和实质及其组成细胞类型进行了定量描述。对甲基丙烯酸乙二醇酯切片进行点计数形态计量学，估计基质和实质的体积分区。静脉占基质的85%，而动脉和胆管各占约6 - 7%。实质占肝脏体积的95%。对透射电子显微镜照片进行点计数形态计量学，估计实质成分的体积分区以及数量和表面密度测量值。在肝实质分区内，肝细胞占85%，并表现出显著的性别差异。雌性肝细胞数量明显更多，但体积较小，仅为雄性肝细胞体积的60%。由于两性的肝细胞核体积相等，差异是由于雌性细胞质体积减小所致。雌性的窦周巨噬细胞在各自的实质分区中占据更大的体积，其较大的平均细胞质体积表明其处于激活状态。小叶前胆管和小叶内胆管的胆管上皮细胞数量众多。肝细胞与胆管上皮细胞的数量密度比与肝细胞的管状排列一致。讨论了可能介导这种性别二态性的因素。
