人β-N-乙酰己糖胺酶的中间形式对4-甲基伞形酮基β-N-乙酰氨基葡萄糖苷6-硫酸盐缺乏活性。
Intermediate forms of human beta-N-acetylhexosaminidase lack activity towards 4-methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate.
作者信息
Beccari T, Emiliani C, Hosseini R, Orlacchio A, Stirling J L
机构信息
Istituto di Chimica Biologica, Facoltà di Scienze MM.FF.NN, Università di Perugia, Italy.
出版信息
Biochem J. 1987 Jun 15;244(3):801-4. doi: 10.1042/bj2440801.
Abstract
4-Methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate was purified from a mixture containing its unsulphated precursor. The substrate was used to test for the presence of functional alpha-subunits in 'intermediate' forms of human beta-N-acetylhexosaminidase in samples of normal and pregnancy serum and in extracts of placenta and lymphocytes from a patient with common acute lymphoblastic leukaemia. Intermediate forms in these samples had no activity towards 4-methylumbelliferyl beta-N-acetylglucosaminide 6-sulphate, indicating that they lack alpha-subunits.
摘要
从含有其未硫酸化前体的混合物中纯化出4-甲基伞形酮基β-N-乙酰氨基葡萄糖苷6-硫酸盐。该底物用于检测正常血清和妊娠血清样本以及一名普通急性淋巴细胞白血病患者的胎盘和淋巴细胞提取物中人类β-N-乙酰己糖胺酶“中间”形式中功能性α亚基的存在。这些样本中的中间形式对4-甲基伞形酮基β-N-乙酰氨基葡萄糖苷6-硫酸盐没有活性，表明它们缺乏α亚基。
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