Strauss Mariana, Velázquez López Daniela A, Moya Diego M, Bazán P Carolina, Báez Alejandra L, Rivarola H Walter, Paglini-Oliva Patricia A, Lo Presti M Silvina
Instituto de Investigaciones en Ciencias de la Salud (INICSA) UNC - CONICET, Centro de Estudios e Investigación de la Enfermedad de Chagas y Leishmaniasis, Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Córdoba, Argentina.
Instituto de Investigaciones en Ciencias de la Salud (INICSA) UNC - CONICET, Centro de Estudios e Investigación de la Enfermedad de Chagas y Leishmaniasis, Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Córdoba, Argentina.
Mol Biochem Parasitol. 2018 Jun;222:29-33. doi: 10.1016/j.molbiopara.2018.04.007. Epub 2018 Apr 27.
In the present work, we evaluated the effect of mixed Trypanosoma cruzi infections, studying the biological distribution of the different parasites in blood, heart and skeletal muscle during the acute phase. Albino Swiss mice were infected with different parasite strain/isolates or with a combination of them. The parasites in the different tissues were typified through specific PCR, population variability was analyzed through RFLP studies and parasitological and histopathological parameters were evaluated. We found a predominance of TcII and TcVI in all tissues samples respect to TcV and different parasite populations were found in circulation and in the tissues from the same host. These results verify the distribution of parasites in host tissues from early stages of infection and show biological interactions among different genotypes and populations of T. cruzi.
在本研究中，我们评估了克氏锥虫混合感染的影响，研究了急性期不同寄生虫在血液、心脏和骨骼肌中的生物学分布。将白化瑞士小鼠感染不同的寄生虫菌株/分离株或它们的组合。通过特异性PCR对不同组织中的寄生虫进行分型，通过RFLP研究分析群体变异性，并评估寄生虫学和组织病理学参数。我们发现，相对于TcV，在所有组织样本中TcII和TcVI占主导地位，并且在同一宿主的循环系统和组织中发现了不同的寄生虫群体。这些结果证实了感染早期寄生虫在宿主组织中的分布，并显示了克氏锥虫不同基因型和群体之间的生物学相互作用。
