Schulz T F, Mitterer M, Neumayer H P, Vogetseder W, Dierich M P
Institute for Hygiene, Innsbruck.
Eur J Immunol. 1988 Aug;18(8):1253-8. doi: 10.1002/eji.1830180816.
A monoclonal antibody, 7F7, directed at a recently described membrane activation antigen of 85 kDa was found to inhibit the T cell proliferation induced by an anti-CD3 antibody, phytohemagglutinin A and concanavalin A. The T cell response to allogenic stimulator cells was also weakly inhibited. The inhibition of these T cell responses was only obtained if the antibody was added within the first 8 h (first 24 h in the case of the concanavalin A response) of culture. In addition the antibody inhibited the formation of cellular aggregates seen in stimulated cultures when added within the first 8 h. The membrane glycoprotein recognized by 7F7 is shown to have a slightly different molecular mass on cells of different lineage, a protein core of 55 kDa and to contain 30-50% of N-linked as well as a small amount of O-linked carbohydrates and sialic acid residues. This study suggests that the highly glycosylated membrane activation antigen defined by antibody 7F7 could contribute to the contact between those cells which are involved in the initiation of T cell responses.
一种针对最近描述的85 kDa膜激活抗原的单克隆抗体7F7，被发现可抑制由抗CD3抗体、植物血凝素A和刀豆球蛋白A诱导的T细胞增殖。T细胞对同种异体刺激细胞的反应也受到微弱抑制。只有在培养的最初8小时内（刀豆球蛋白A反应的情况下为最初24小时）加入该抗体，才能抑制这些T细胞反应。此外，当在最初8小时内加入时，该抗体可抑制在刺激培养物中观察到的细胞聚集体的形成。7F7识别的膜糖蛋白在不同谱系的细胞上显示出略有不同的分子量，其蛋白核心为55 kDa，含有30-50%的N-连接以及少量的O-连接碳水化合物和唾液酸残基。这项研究表明，由抗体7F7定义的高度糖基化膜激活抗原可能有助于参与T细胞反应起始的那些细胞之间的接触。
