La Clair James J
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037 (USA), Fax: Int. code+(1) 619 784-2584.
Angew Chem Int Ed Engl. 1998 Feb 16;37(3):325-329. doi: 10.1002/(SICI)1521-3773(19980216)37:3<325::AID-ANIE325>3.0.CO;2-L.
Single biomolecules can be detected with the aid of new labels that absorb visible light and fluoresce by intramolecular charge transfer. The intensity of the fluorescence is highly dependent upon the environment of the fluorophore, and decreases greatly with increasing polarity.
借助能吸收可见光并通过分子内电荷转移发出荧光的新型标记物，可以检测单个生物分子。荧光强度高度依赖于荧光团的环境，并且随着极性的增加而大大降低。
