免疫检查点分子CD200与膀胱癌的肿瘤分级和转移相关。

The Immune Checkpoint Molecule CD200 Is Associated with Tumor Grading and Metastasis in Bladder Cancer.

作者信息

Rexin Peter, Tauchert Alessa, Hänze Jörg, Heers Hendrik, Schmidt Ansgar, Hofmann Rainer, Hegele Axel

机构信息

Institute of Pathology, University Medical Center Marburg, Marburg, Germany.

Department of Urology and Pediatric Urology, University Medical Center Marburg, Marburg, Germany.

出版信息

Anticancer Res. 2018 May;38(5):2749-2754. doi: 10.21873/anticanres.12517.

DOI:10.21873/anticanres.12517

PMID:29715095

Abstract

BACKGROUND

We examined the expression of CD200, a ligand of immune tolerance, in transitional cell carcinoma of the human bladder (TCC).

MATERIALS AND METHODS

CD200 was analyzed by immunohistochemistry (IHC) in 90 patients with suspected TCC lesions of the bladder. Expression of CD200 was exemplarily validated by quantitative reverse transcription polymerase chain reaction and western blot analysis.

RESULTS

CD200 was detectable at mRNA and protein levels in TCC homogenate and TCC cell lines (T24, UMUC3). TCC tissues showed significantly higher CD200 expression (p<0.005) than normal bladder tissues. CD200 signals were also higher in metastasized compared to localized TCC (p<0.05). CD200 was significantly correlated to tumor grading (p<0.001) and was strongest in the subgroup with high-grade G2 TCC (vs. low-grade G2 p<0.05).

CONCLUSION

This is the first report of CD200 expression in patients with TCC. The significant correlation between CD200 expression and tumor grading may suggest CD200 as a potential target and marker for immunotherapeutic approaches.

摘要

背景

我们检测了免疫耐受配体CD200在人膀胱移行细胞癌（TCC）中的表达。

材料与方法

采用免疫组织化学（IHC）方法对90例疑似膀胱TCC病变患者的CD200进行分析。通过定量逆转录聚合酶链反应和蛋白质印迹分析对CD200的表达进行了示例性验证。

结果

在TCC匀浆和TCC细胞系（T24、UMUC3）中可检测到CD200的mRNA和蛋白水平。TCC组织中CD200的表达明显高于正常膀胱组织（p<0.005）。与局限性TCC相比，转移性TCC中的CD200信号也更高（p<0.05）。CD200与肿瘤分级显著相关（p<0.001），在高级别G2 TCC亚组中最强（与低级别G2相比，p<0.05）。