The acid tolerance response and pH adaptation of in extract of lime from Aceh Indonesia.

The objective of the present study was to evaluate the acid tolerance response and pH adaptation when interacted with extract of lime ( ). We used ATCC 29212 and lime extract from Aceh, Indonesia. The microbe was analyzed for its pH adaptation, acid tolerance response, and adhesion assay using a light microscope with a magnification of x1000. Further, statistical tests were performed to analyze both correlation and significance of the acid tolerance and pH adaptation as well as the interaction activity. was able to adapt to a very acidic environment (pH 2.9), which was characterized by an increase in its pH (reaching 4.2) at all concentrations of the lime extract (p < 0.05). was also able to provide acid tolerance response to lime extract based on spectrophotometric data (595 nm) (p < 0.05). Also, the interaction activity of in different concentrations of lime extract was relatively stable within 6 up to 12 hours (p < 0.05), but it became unstable within 24-72 hours (p > 0.05) based on the mass profiles of its interaction activity. can adapt to acidic environments (pH 2.9-4.2); it is also able to tolerate acid generated by extract, revealing a stable interaction in the first 6-12 hours.