Department of Ophthalmology, College of Medicine, University of Florida Health Science Center, Gainesville, FL, USA.
Adv Exp Med Biol. 2018;1074:479-484. doi: 10.1007/978-3-319-75402-4_59.
Müller cells provide support to photoreceptors under many conditions of stress and degeneration. Leukemia inhibitory factor is known to be expressed in Müller cells, which is necessary to promote photoreceptor survival in stress. We hypothesize that Müller cells that express LIF are undergoing other biological processes or functions which may benefit photoreceptors in disease. In this study, we analyze an existing single Müller cell microarray dataset to determine which processes are upregulated in Müller cells that express LIF, by correlating LIF expression to the expression of other genes using a robust correlation method. Some enriched processes include divalent inorganic cation homeostasis, negative regulation of stem cell proliferation, and gamma-glutamyl transferase activity.
Müller 细胞在多种应激和变性条件下为光感受器提供支持。已知白血病抑制因子在 Müller 细胞中表达，这对于促进应激状态下光感受器的存活是必要的。我们假设表达 LIF 的 Müller 细胞正在经历其他生物学过程或功能，这可能有益于疾病中的光感受器。在这项研究中，我们通过使用稳健的相关方法，将 LIF 表达与其他基因的表达相关联，分析现有的单个 Müller 细胞微阵列数据集，以确定表达 LIF 的 Müller 细胞中哪些过程被上调。一些富集的过程包括二价无机阳离子稳态、干细胞增殖的负调控和谷氨酰转移酶活性。
