Pan Yuanhu, Zhang Heying, Xi Chenglong, Huang Lingli, Xie Shuyu, Chen Dongmei, Tao Yanfei, Liu Zhenli, Yuan Zonghui
National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei, China.
MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei, China.
Biomed Chromatogr. 2018 May 2:e4268. doi: 10.1002/bmc.4268.
A simple and reliable LC-MS/MS method was established for simultaneous determination of 12 components from acetylkitasamycin and kitasamycin in swine plasma. The analytes were separated on a Shim-pack VP-ODS column with a 25 min gradient elution using 5 mmol/L ammonium acetate and acetonitrile as the mobile phase at a flow rate of 0.2 mL/min. Identification and quantification were accomplished by electrospray ionization) in positive mode using multiple reaction monitoring. The limits of quantitation of acetylkitasamycin A A , A and kitasamycin A , A were 3 μg/L, and that of the other eight components was 5 μg/L. The mean recoveries of kitasamycin and acetylkitasamycin ranged from 85.3 to 103.5%. The developed method was successfully applied to a pharmacokinetic study in swine after intravenous (i.v.) and oral (p.o.) administration of acetylkitasamycin. The result showed that the plasma concentrations of acetylkitsamycin components were much higher than that of kitasamycin in swine after i.v. and p.o., in which acetylkitsamycin A A was the highest component at each time point.
建立了一种简单可靠的液相色谱-串联质谱法，用于同时测定猪血浆中乙酰吉他霉素和吉他霉素的12种成分。采用Shim-pack VP-ODS柱，以5 mmol/L醋酸铵和乙腈为流动相，流速为0.2 mL/min，进行25 min梯度洗脱，分离分析物。采用电喷雾电离正离子模式和多反应监测进行定性和定量分析。乙酰吉他霉素A、A、A和吉他霉素A、A的定量限为3 μg/L，其他8种成分的定量限为5 μg/L。吉他霉素和乙酰吉他霉素的平均回收率为85.3%至103.5%。所建立的方法成功应用于乙酰吉他霉素静脉注射（i.v.）和口服（p.o.）给药后猪的药代动力学研究。结果表明，静脉注射和口服后，猪血浆中乙酰吉他霉素成分的浓度远高于吉他霉素，其中乙酰吉他霉素A在各时间点含量最高。
