a Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE) , Faisalabad , Pakistan.
Int J Phytoremediation. 2018 Jun 7;20(7):675-681. doi: 10.1080/15226514.2017.1413331.
Phytoremediation is a promising approach for the cleanup of soil contaminated with petroleum hydrocarbons. This study aimed to develop plant-bacterial synergism for the successful remediation of crude oil-contaminated soil. A consortia of three endophytic bacteria was augmented to two grasses, Leptochloa fusca and Brachiaria mutica, grown in oil-contaminated soil (46.8 g oil kg soil) in the vicinity of an oil exploration and production company. Endophytes augmentation improved plant growth, crude oil degradation, and soil health. Maximum oil degradation (80%) was achieved with B. mutica plants augmented with the endophytes and it was significantly (P < 0.05) higher than the use of plants or bacteria individually. Moreover, endophytes showed more persistence, the abundance and expression of alkB gene in the rhizosphere as well as in the endosphere of the tested plants than in unvegetated soil. A positive relationship (r = 0.70) observed between gene expression and crude oil reduction indicates that catabolic gene expression is important for hydrocarbon mineralization. This investigation showed that the use of endophytes with appropriate plant is an effective strategy for the cleanup of oil-contaminated soil under field conditions.
植物修复是一种很有前途的方法，可以用于清理受到石油碳氢化合物污染的土壤。本研究旨在开发植物-细菌协同作用，以成功修复受原油污染的土壤。将三株内生菌与两种在附近的石油勘探和生产公司的受污染土壤（46.8 g 油 kg 土壤）中生长的草（Leptochloa fusca 和 Brachiaria mutica）进行了共培养。内生菌的添加提高了植物的生长、原油的降解和土壤的健康状况。与单独使用植物或细菌相比，用内生菌添加到 B. mutica 植物中可实现最大的石油降解（80%），且具有显著差异（P < 0.05）。此外，内生菌在根际和测试植物的内围中比在无植被土壤中表现出更高的持久性、alkB 基因的丰度和表达。观察到基因表达与原油减少之间存在正相关关系（r = 0.70），表明代谢基因表达对于烃类矿化很重要。这项研究表明，在田间条件下，使用适当的植物与内生菌结合是一种清理受污染土壤的有效策略。
