Wang Yong Qiang, Liu Zi Hou, Shu Jie, Tang Shen E, Luo Fan Yan
Department of Cardiac Surgery, Central South University,Changsha 410008,China.
Institute of Medical Sciences,Xiangya Hospital,Central South University,Changsha 410008,China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2018 Apr 28;40(2):219-224. doi: 10.3881/j.issn.1000-503X.2018.02.012.
Objective To explore the role of Galectin3 in transforming growth factor-β(TGF-β)-induced epithelial-mesenchymal transition (EMT) in A549 cells. Methods Galectin3 was over-expressed in an A549 cell line. EMT was induced in lung cancer A549 cells by adding TGF-β. The expressions of Galectin3,E-cadherin,and vimentin were determined by Western blot. The protein expression of E-cadherin and the morphological changes of the cells were detected by immunofluorescence. Cellular proliferation was analyzed with cell counting kit-8,and the cellular migration and invasion was measured by scratches healing and Transwell assay,respectively.Results When only Galectin3 was over-expressed in A549 cell line,the expression levels of EMT-related proteins such as E-cadherin and vimentin were not changed,and the abilities of cellular proliferation,invasion,and migration were not changed either. When the EMT was induced by TGF-β in A549 cells,the E-cadherin expression was down-regulated and the vimentin expression was up-regulated in A549 cells with Galectin3 over-expression. There was no significant change in cellular proliferation,whereas the abilities of cellular invasion and migration were enhanced.Conclusion The TGF-β-induced EMT in A549 cells can be enhanced by Galectin3.
目的 探讨半乳糖凝集素3在转化生长因子-β（TGF-β）诱导A549细胞上皮-间质转化（EMT）中的作用。方法 在A549细胞系中过表达半乳糖凝集素3。通过添加TGF-β诱导肺癌A549细胞发生EMT。采用蛋白质免疫印迹法检测半乳糖凝集素3、E-钙黏蛋白和波形蛋白的表达。通过免疫荧光检测E-钙黏蛋白的蛋白表达及细胞形态变化。使用细胞计数试剂盒-8分析细胞增殖情况，分别采用划痕愈合实验和Transwell实验检测细胞迁移和侵袭能力。结果 仅在A549细胞系中过表达半乳糖凝集素3时，E-钙黏蛋白和波形蛋白等EMT相关蛋白的表达水平未发生改变，细胞增殖、侵袭和迁移能力也未改变。当TGF-β诱导A549细胞发生EMT时，过表达半乳糖凝集素3的A549细胞中E-钙黏蛋白表达下调，波形蛋白表达上调。细胞增殖无明显变化，而细胞侵袭和迁移能力增强。结论 半乳糖凝集素3可增强TGF-β诱导的A549细胞EMT。
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