Yang Lei, Zhang Zhen-Hai, Jia Xiao-Bin
College of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Zhongguo Zhong Yao Za Zhi. 2018 Apr;43(7):1446-1452. doi: 10.19540/j.cnki.cjcmm.20180117.002.
Traditional Chinese medicine combined with anticancer drugs is a new direction of clinical cancer therapy in recent years. In this study, the optimal ratio of ginseng rare ginsenoside components and paclitaxel was optimized by MTT method, and the proliferative, apoptotic and anti-tumor effects of lung cancer A549 cells were investigated. It was found that the inhibitory effect on the proliferation of lung cancer A549 cells was the same as that on paclitaxel when the ratio of rare ginseng rare ginsenoside components to paclitaxel was 4∶6. Further studies showed that the combined therapy significantly increased the inductive effect of apoptosis in A549 cells, and up-regulated the expression of caspase-3 protein and down-regulated the ratio of Bcl-2/Bax. The tumor-bearing mice model showed that the combination therapy of ginseng rare ginsenoside components and paclitaxel could significantly inhibit the growth of tumor and alleviate the toxic and side effects of paclitaxel on liver. A multi-component system of ginseng rare ginsenoside components-paclitaxel was established in this paper. The proliferation and growth of lung cancer A549 cells were inhibited by paclitaxel-induced apoptosis, the dosage of paclitaxel and the toxicity of paclitaxel were reduced, and the effect of anti-lung cancer was enhanced, which provided a theoretical basis for later studies and clinical application.
中药与抗癌药物联合应用是近年来临床肿瘤治疗的新方向。本研究采用MTT法优化人参稀有皂苷成分与紫杉醇的最佳配比，并研究其对肺癌A549细胞的增殖、凋亡及抗肿瘤作用。结果发现，当稀有皂苷成分与紫杉醇的比例为4∶6时，对肺癌A549细胞增殖的抑制作用与紫杉醇相当。进一步研究表明，联合治疗显著增强了A549细胞凋亡的诱导作用，上调了caspase-3蛋白的表达，下调了Bcl-2/Bax比值。荷瘤小鼠模型显示，人参稀有皂苷成分与紫杉醇联合治疗可显著抑制肿瘤生长，减轻紫杉醇对肝脏的毒副作用。本文建立了人参稀有皂苷成分-紫杉醇多组分体系。紫杉醇诱导凋亡抑制肺癌A549细胞的增殖和生长，降低了紫杉醇的用量和毒性，增强了抗肺癌作用，为后续研究和临床应用提供了理论依据。
