Salman Doaa, Pumidonming Wilawan, Oohashi Eiji, Igarashi Makoto
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Department of Animal Medicine, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt.
J Vet Med Sci. 2018 Jun 29;80(6):960-967. doi: 10.1292/jvms.17-0713. Epub 2018 May 2.
The present study investigated the prevalence of Toxoplasma gondii and other intestinal parasites in cats in the Tokachi subprefecture in Japan. A total of 365 household cats were included in the study, and 353 serum and 351 fecal samples were collected and analyzed. T. gondii IgG antibodies were detected in the sera of 16.14% of cats based on Latex agglutination test and ELISA. For ELISA, T. gondii RH strain tachyzoites lysate and T. gondii SAG2 recombinant protein were used as antigens. Low seropositivity was detected in cats younger than one year and older than 11 years; outdoor and hunter cats showed significantly high seropositivities. Neutering either in male or female cats, but not gender, had a considerable effect on seroprevalence. Toxoplasma gondii oocysts were detected in one fecal sample. The overall parasitic infestation in cats was 12.5%. Other detected parasites included Toxocara species, which showed the highest prevalence of 7.7%, followed by Isospora spp. (2%), Taenia spp. (1.7%), and Ancylostoma spp. (0.9%). Spirometra spp. was detected in only one sample. Outdoor cats comprised 50% of all 44 parasite-infested cats. Although T. gondii oocysts were detected in only one sample, the relatively high seroprevalence of T. gondii indicated that it can pose significant risks to the environment. Our findings highlighted the potential of outdoor cats as a source of T. gondii and other parasites.
本研究调查了日本十胜地区家猫中弓形虫及其他肠道寄生虫的感染率。共有365只家猫纳入本研究，采集并分析了353份血清样本和351份粪便样本。基于乳胶凝集试验和酶联免疫吸附测定法（ELISA），在16.14%的猫血清中检测到了弓形虫IgG抗体。对于ELISA，使用弓形虫RH株速殖子裂解物和弓形虫SAG2重组蛋白作为抗原。在1岁以下和11岁以上的猫中检测到较低的血清阳性率；户外猫和捕猫的血清阳性率显著较高。对雄性或雌性猫进行绝育，但不是性别，对血清阳性率有相当大的影响。在一份粪便样本中检测到了弓形虫卵囊。猫的总体寄生虫感染率为12.5%。其他检测到的寄生虫包括弓首蛔虫属，其感染率最高，为7.7%，其次是等孢球虫属（2%）、带绦虫属（1.7%）和钩口线虫属（0.9%）。仅在一份样本中检测到裂头绦虫属。户外猫占所有44只寄生虫感染猫的50%。尽管仅在一份样本中检测到弓形虫卵囊，但弓形虫相对较高的血清阳性率表明它可能对环境构成重大风险。我们的研究结果突出了户外猫作为弓形虫和其他寄生虫来源的可能性。
