Li J Z, Lian E C
Veterans Administration Medical Center, Miami, Florida 33125.
Thromb Haemost. 1988 Jun 16;59(3):432-4.
The effects of acidic mucopolysaccharide extracted from sea cucumber (Stichopus japonicus Selenka) (SJAMP) on rabbit platelets were studied. Using citrated platelet-rich plasma (PRP), washed platelets, and formaldehyde fixed platelets from 10 New Zealand white rabbits, we investigated the effects of platelet inhibitors and various plasma and its fractions on SJAMP-induced agglutination. It was found that the tracing of platelet agglutination induced by SJAMP showed a single phase without a lag period. The lowest concentration of SJAMP required for the agglutination of rabbit platelets was approximately 2 micrograms/ml, and the magnitude of agglutination induced by SJAMP was concentration dependent. In 8 out of 10 rabbits, the platelets in PRP were agglutinated by 10 micrograms/ml of SJAMP. Platelet inhibitors, such as aspirin, indomethacin, apyrase, antimycin, 2-deoxy-D-glucose and EDTA did not inhibit the agglutination induced by SJAMP. Washed rabbit platelets were not agglutinated by SJAMP even though the concentration of SJAMP was raised up to 50 micrograms/ml. When rabbit plasma, serum, or 50-60% ammonium sulfate saturated plasma fraction was added to the reaction mixture, agglutination of washed platelets by SJAMP was recovered completely. But human plasma or fibrinogen did not have any effect on the reactivity of washed rabbit platelets to SJAMP. From these data we conclude that the SJAMP-induced rabbit platelet agglutination is independent of energy metabolism but requires plasma cofactor(s) other than fibrinogen. The plasma cofactor is present in 50-60% ammonium sulfate saturated plasma fraction.
研究了从海参（刺参）中提取的酸性粘多糖（SJAMP）对兔血小板的影响。使用来自10只新西兰白兔的枸橼酸化富血小板血浆（PRP）、洗涤血小板和甲醛固定血小板，我们研究了血小板抑制剂以及各种血浆及其组分对SJAMP诱导的凝集作用的影响。结果发现，SJAMP诱导的血小板凝集追踪显示为单相，无滞后阶段。兔血小板凝集所需的SJAMP最低浓度约为2微克/毫升，且SJAMP诱导的凝集程度呈浓度依赖性。在10只兔子中的8只，10微克/毫升的SJAMP可使PRP中的血小板凝集。血小板抑制剂，如阿司匹林、吲哚美辛、腺苷三磷酸双磷酸酶、抗霉素、2-脱氧-D-葡萄糖和乙二胺四乙酸，均不抑制SJAMP诱导的凝集。即使将SJAMP浓度提高到50微克/毫升，洗涤后的兔血小板也不会被SJAMP凝集。当向反应混合物中加入兔血浆、血清或50-60%硫酸铵饱和血浆组分时，SJAMP对洗涤血小板的凝集作用完全恢复。但人血浆或纤维蛋白原对洗涤后的兔血小板对SJAMP的反应性没有任何影响。根据这些数据我们得出结论，SJAMP诱导的兔血小板凝集与能量代谢无关，但需要除纤维蛋白原之外的血浆辅因子。该血浆辅因子存在于50-60%硫酸铵饱和血浆组分中。
