Nakhaie Mohsen, Soleimanjahi Hoorieh, Mollaie Hamid Reza, Arabzadeh Seyed Mohamad Ali
Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Dept. of Medical Microbiology, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Pathol. 2018 Winter;13(1):54-62.
Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. The Multiplex RT PCR method was the preferred method for the detection of influenza A, B, and adenoviruses in clinical specimens because it is rapid, sensitive, specific, and more cost-effective than alternative methods.
After collecting samples from patients with respiratory disease, virus genome was extracted, then Monoplex PCR was used on positive samples and Multiplex RT-PCR on clinical specimens. Finally, by comparing the bands of these samples, the type of virus in the clinical samples was determined.
Performing Multiplex RT-PCR on 50 samples of respiratory tract led to following results; flu A: 12.5%, fluB: 50%, adeno: 27.5%, negative: 7.5%, and 2.5% contamination.
Reverse transcription-multiplex Polymerase Chain Reaction (PCR) technique, a rapid diagnostic tool, has potential for high-throughput testing. This method has a significant advantage, which provides simultaneous amplification of numerous viruses in a single reaction. This study concentrates on multiplex molecular technologies and their clinical application for the detection and quantification of respiratory pathogens. The improvement in diagnostic testing for viral respiratory pathogens effects patient management, and leads to more cost-effective delivery of care. It limits unnecessary antibiotic use and improves clinical management by use of suitable treatment.
发展中国家数以百万计的人死于急性呼吸道感染，如甲型和乙型流感病毒以及腺病毒。鉴于快速鉴定病毒的重要性，在本研究中，研究人员试图设计一种能够快速同时检测甲型、乙型流感病毒和腺病毒的方法。多重逆转录聚合酶链反应（Multiplex RT PCR）方法是检测临床标本中甲型、乙型流感病毒和腺病毒的首选方法，因为它快速、灵敏、特异，且比其他方法更具成本效益。
从呼吸道疾病患者采集样本后，提取病毒基因组，然后对阳性样本进行单重PCR，对临床标本进行多重逆转录聚合酶链反应。最后，通过比较这些样本的条带，确定临床样本中的病毒类型。
对50份呼吸道样本进行多重逆转录聚合酶链反应，结果如下：甲型流感：12.5%，乙型流感：50%，腺病毒：27.5%，阴性：7.5%，污染：2.5%。
逆转录 - 多重聚合酶链反应（PCR）技术作为一种快速诊断工具，具有高通量检测的潜力。该方法具有显著优势，能够在单个反应中同时扩增多种病毒。本研究聚焦于多重分子技术及其在呼吸道病原体检测和定量中的临床应用。病毒呼吸道病原体诊断检测的改进影响患者管理，并带来更具成本效益的医疗服务。它限制了不必要的抗生素使用，并通过采用适当治疗改善临床管理。
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