Huang Ru, Gao Lei
Department of Heart Failure, Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.
Key Laboratory of Arrhythmias of The Ministry of Education of China, Research Institute of Heart Failure, Shanghai East Hospital, Dalian Medical University, Shanghai 200120, P.R. China.
Oncol Lett. 2018 May;15(5):6436-6442. doi: 10.3892/ol.2018.8153. Epub 2018 Mar 1.
Non-small cell lung cancer (NSCLC) is the most commonly diagnosed subtype of lung cancer, and the leading cause of cancer-associated mortalities worldwide. However, NSCLC is typically diagnosed at a late stage of disease due to a lack of effective diagnostic methods. In the present study, the GSE19804 dataset was obtained from the Gene Expression Omnibus, and a number of differentially expressed genes were identified between NSCLC and adjacent normal tissues. Based on functional and pathway enrichment analyses, five hub genes (cell-division cycle 20, centromere protein F, kinesin family member 2C, BUB1 mitotic checkpoint serine/threonine kinase and ZW10 interacting kinetochore protein) were selected. After verifying that the mRNA level of these hub genes was also upregulated in NSCLC tissues by using the GSE10072 dataset and in cell lines by reverse transcription-quantitative polymerase chain reaction. The diagnostic and prognostic potentials of these five gene candidates were evaluated using receiver operating characteristic curves and survival analyses. Taken together, the present study identified five candidates that are overexpressed in NSCLC tissues and could also serve as potential diagnostic and prognostic biomarkers for patients with NSCLC.
非小细胞肺癌（NSCLC）是最常见的肺癌诊断亚型，也是全球癌症相关死亡的主要原因。然而，由于缺乏有效的诊断方法，NSCLC通常在疾病晚期才被诊断出来。在本研究中，从基因表达综合数据库中获取了GSE19804数据集，并鉴定出NSCLC与相邻正常组织之间的一些差异表达基因。基于功能和通路富集分析，选择了五个核心基因（细胞分裂周期20、着丝粒蛋白F、驱动蛋白家族成员2C、BUB1有丝分裂检查点丝氨酸/苏氨酸激酶和ZW10相互作用动粒蛋白）。通过使用GSE10072数据集以及通过逆转录-定量聚合酶链反应在细胞系中验证这些核心基因的mRNA水平在NSCLC组织中也上调后，使用受试者工作特征曲线和生存分析评估了这五个候选基因的诊断和预后潜力。综上所述，本研究鉴定出五个在NSCLC组织中过表达的候选基因，它们也可作为NSCLC患者潜在的诊断和预后生物标志物。
