State Key Laboratory of Powder Metallurgy, Central South University, Changsha, 410083, People's Republic of China.
Department of Anesthesiology, The Second Xiang Ya Hospital, Central South University, Changsha, 410011, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2019 Apr;107(3):573-581. doi: 10.1002/jbm.b.34149. Epub 2018 May 7.
By combining the biocompatibility of Ta and excellent performance of Nb, the β-type Ta-xNb (x = 5, 10, and 15 wt %) alloys with three-dimensional network structure was successfully fabricated. This work was designed to carry out the systematic study of in vitro/vivo biocompatibility of highly porous Ta-Nb alloys. In the present study, the in vitro biological response was characterized by cell attachment, cytotoxicity test, and gene expression. Cells were well distributed on porous alloy surface and both bulk/porous Ta-15Nb alloys showd a 0-1 RGR level. Moreover, the upregulated gene expression may reveal an increase in bone regeneration. An assessment of primary osteogenic response in vivo was achieved by histological evaluation and pull-out test. In vivo results demonstrated that the porous Ta-15Nb alloy showed a higher pull-out force than bulk Ta-15Nb alloy and exhibited a more extensive contact between implants and bones after 12 weeks. Based on the present experiments, porous Ta-Nb alloy scaffolds had a good biocompatibility and were promising for biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 573-581, 2019.
通过结合 Ta 的生物相容性和 Nb 的优异性能，成功制备出具有三维网络结构的β型 Ta-xNb（x=5、10 和 15wt%）合金。这项工作旨在对高多孔 Ta-Nb 合金的体外/体内生物相容性进行系统研究。在本研究中，通过细胞附着、细胞毒性试验和基因表达来表征体外生物反应。细胞在多孔合金表面均匀分布，块状/多孔 Ta-15Nb 合金均表现出 0-1 RGR 水平。此外，上调的基因表达可能表明骨再生增加。通过组织学评价和拔出试验对体内原发性成骨反应进行评估。体内结果表明，多孔 Ta-15Nb 合金的拔出力高于块状 Ta-15Nb 合金，并且在 12 周后植入物与骨骼之间具有更广泛的接触。基于目前的实验，多孔 Ta-Nb 合金支架具有良好的生物相容性，有望用于生物医学应用。©2018Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，2018。©2018Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 107B：573-581，2019。
