赤狐（ Vulpes vulpes）体内刚地弓形虫、犬新孢子虫和兔脑炎微孢子虫的分子证据
Molecular Evidence of Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi in Red Foxes ( Vulpes vulpes).
作者信息
Lukášová Radka, Marková Jiřina, Bártová Eva, Murat Jean-Benjamin, Sedlák Kamil
机构信息
1 Faculty of Veterinary Hygiene and Ecology, Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences, Palackého tř. 1946/1, 612 42 Brno, Czech Republic.
2 INSERM, UMR S 1094, Neuroépidémiologie Tropicale, Université de Limoges, 87025 Limoges, France.
出版信息
J Wildl Dis. 2018 Oct;54(4):825-828. doi: 10.7589/2017-09-240. Epub 2018 May 7.
Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi are important infectious agents, with T. gondii and E. cuniculi having zoonotic potential. There are two main clonal lineages (types I and II) of T. gondii in Europe, but little is known about genotypes of T. gondii in wild animals. The aim of our study was molecular detection of these three pathogens in tissues of wild red foxes ( Vulpes vulpes) from the Czech Republic. Using PCR (B1 gene), we detected T. gondii in 10% of the animals that we tested ( n=100); N. caninum and E. cuniculi were not detected. The T. gondii samples were genotyped by single multiplex PCR assay with 15 microsatellite markers. Five samples were successfully genotyped as genotype II, a unique finding for T. gondii isolated from red foxes from the Czech Republic.
刚地弓形虫、犬新孢子虫和兔脑炎微孢子虫是重要的感染因子，其中刚地弓形虫和兔脑炎微孢子虫具有人畜共患病潜力。在欧洲，刚地弓形虫有两个主要的克隆谱系（I型和II型），但对于野生动物中刚地弓形虫的基因型了解甚少。我们研究的目的是对来自捷克共和国的野生赤狐（赤狐属）组织中的这三种病原体进行分子检测。通过PCR（B1基因），我们在检测的100只动物中有10%检测到了刚地弓形虫；未检测到犬新孢子虫和兔脑炎微孢子虫。通过使用含有15个微卫星标记的单重多重PCR分析法对刚地弓形虫样本进行基因分型。五个样本成功基因分型为II型，这对于从捷克共和国赤狐中分离出的刚地弓形虫来说是一个独特的发现。
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