[肾癌细胞中缺氧诱导因子抑制因子的生长调控]

[Growth Regulation of Factor Inhibiting Hypoxia-Inducible Factor in Renal Carcinoma Cells].

作者信息

Deng Shi, Yang Bo, Ren Zheng-Ju, Dong Qiang

机构信息

Department of Urology,Institute of Urology,West China Hospital,Sichuan University,Chengdu 610041,China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 Jan;49(1):29-33.

PMID:29737085

Abstract

OBJECTIVE

To detect the expression and growth regulation effects of the factor inhibiting hypoxia-inducible factor (FIH-1) in renal carcinoma cells.

METHODS

The expressions of FIH-1,hypoxia inducible factor (HIF) and vascular endothelial growth factor (VEGF) were examined in OS-RC-2 and 786-0 cell lines via real-time PCR (RT-PCR) and Western blot. Overexpression plasmid of were designed and constructed as EX-V1104-M45- After the transfection of the plasmid into OS-RC-2 and 786-0 cells,the expression levels of FIH-1,HIF and VEGF were measured by RT-PCR and Western blot,while cell proliferation was tested by MTT.

RESULTS

The expressions of FIH-1,HIF-1α,HIF-2α and VEGF were detected in OS-RC-2 cells,and FIH-1,HIF-2α and VEGF were detected in 786-0 cells. After the transfection of EX-V1104-M45-, the level of FIH-1 was increased in OS-RC-2,while the levels of HIF-2α,HIF-1α and VEGF were decreased. MTT showed that plasmid transfection decreased the cell proliferation of OS-RC-2,but did not show inhibition on 786-0 cells proliferation.

CONCLUSION

FIH-1/HIF-1α/VEGF signaling pathway exists in renal carcinoma cell lines.

摘要

目的

检测抑制缺氧诱导因子（FIH-1）在肾癌细胞中的表达及生长调控作用。

方法

通过实时荧光定量聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测FIH-1、缺氧诱导因子（HIF）和血管内皮生长因子（VEGF）在OS-RC-2和786-0细胞系中的表达。设计并构建FIH-1过表达质粒EX-V1104-M45-，将该质粒转染至OS-RC-2和786-0细胞后，用RT-PCR和蛋白质免疫印迹法检测FIH-1、HIF和VEGF的表达水平，同时用MTT法检测细胞增殖情况。

结果

在OS-RC-2细胞中检测到FIH-1、HIF-1α、HIF-2α和VEGF的表达，在786-0细胞中检测到FIH-1、HIF-2α和VEGF的表达。转染EX-V1104-M45-后，OS-RC-2细胞中FIH-1水平升高，而HIF-2α、HIF-1α和VEGF水平降低。MTT结果显示，该质粒转染可降低OS-RC-2细胞的增殖，但对786-0细胞的增殖无抑制作用。