Cheng Shaoze, Wang Man, Wang Yingjie, Zhang Chen, Wang Yilin, Song Jiuzhou, Zuo Qisheng, Zhang Yani, Li Bichun
Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, P.R China.
Animal and Avian Sciences, University of Maryland, College Park, Maryland.
J Cell Biochem. 2018 Aug;119(8):6926-6934. doi: 10.1002/jcb.26891. Epub 2018 May 8.
Primitive germ cells (PGCs) are the "ancestor" of sex germ cells. However, their regulation mechanism is not clear at present. The aim of this study is to investigate the effect of PPAR signaling pathway on the differentiation of chicken embryonic stem cells (ESCs) into PGCs, providing theoretical support for the further study of the mechanism of regulation of chicken PGCs. Based on the results of RNA-Seq analysis, the key signal pathway PPAR and the key signal molecule RXRG in the pathway were successfully screened. Also, we made the PPAR signal transduction pathway be activated in vivo and in vitro. The results showed that the expression of FABP3 and SCD-1 was up-regulated by high expression of RXRG, which significantly up-regulated the expression of Cvh, C-kit, and the ratio of Cvh + C-kit+ cells was higher than that of control group. The formation of PGCs was blocked by interfering with PPAR signaling pathway. We conclude that RXRG positively regulates the PPAR signaling pathway and promotes differentiation of chicken ESCs into PGCs. Thus, gene RXRG can be used to study the regulation of PGCs differentiation in chickens.
原始生殖细胞（PGCs）是生殖细胞的“祖先”。然而，其调控机制目前尚不清楚。本研究旨在探讨PPAR信号通路对鸡胚胎干细胞（ESCs）向PGCs分化的影响，为进一步研究鸡PGCs的调控机制提供理论支持。基于RNA-Seq分析结果，成功筛选出关键信号通路PPAR及其通路中的关键信号分子RXRG。此外，我们在体内和体外激活了PPAR信号转导通路。结果显示，RXRG的高表达上调了FABP3和SCD-1的表达，显著上调了Cvh、C-kit的表达，且Cvh+C-kit+细胞的比例高于对照组。干扰PPAR信号通路会阻碍PGCs的形成。我们得出结论，RXRG正向调控PPAR信号通路并促进鸡ESCs向PGCs分化。因此，基因RXRG可用于研究鸡PGCs分化的调控。
