Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Cauerstrasse 6, 91058, Erlangen, Germany.
Department of Materials Engineering and Design, Faculty of Mechanical and Manufacturing, Universiti Tun Hussein Onn Malaysia, 86400, Parit Raja, Batu Pahat, Johor, Malaysia.
Sci Rep. 2018 May 8;8(1):7286. doi: 10.1038/s41598-018-25243-9.
Surface modification of superparamagnetic iron oxide nanoparticles (SPIONs) has been introduced with lauric acid and oleic acid via co-precipitation and thermal decomposition methods, respectively. This modification is required to increase the stability of SPIONs when incorporated in hydrophobic, biodegradable and biocompatible polymers such as poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). In this work, the solid-in-oil-in-water (S/O/W) emulsion-solvent extraction/evaporation method was utilized to fabricate magnetic polymer microspheres incorporating SPIONs in PHBV. The prepared magnetic PHBV microspheres exhibited particle sizes <1 µm. The presence of functional groups of lauric acid, oleic acid and iron oxide in the PHBV microspheres was confirmed by Fourier Transform Infrared spectroscopy (FTIR). X-ray diffraction (XRD) analysis was performed to further confirm the success of the combination of modified SPIONs and PHBV. Thermogravimetric analysis (TGA) indicated that PHBV microspheres were incorporated with SPIONs as compared with SPIONs. This was also proven via magnetic susceptibility measurement as a higher value of this magnetic property was detected for PHBV/SPIONs microspheres. It was revealed that the magnetic PHBV microspheres were non-toxic when assessed with mouse embryotic fibroblast cells (MEF) at different concentrations of microspheres. These results confirmed that the fabricated magnetic PHBV microspheres are potential candidates for use in biomedical applications.
通过共沉淀和热分解法分别用月桂酸和油酸对超顺磁性氧化铁纳米粒子（SPIONs）进行表面改性，以提高 SPIONs 在掺入疏水性、可生物降解和生物相容聚合物（如聚（3-羟基丁酸-co-3-羟基戊酸）（PHBV））时的稳定性。在这项工作中，采用固-油-水（S/O/W）乳液-溶剂萃取/蒸发法制备了含有 PHBV 中 SPIONs 的磁性聚合物微球。所制备的磁性 PHBV 微球的粒径<1μm。傅里叶变换红外光谱（FTIR）证实了 PHBV 微球中存在月桂酸、油酸和氧化铁的官能团。X 射线衍射（XRD）分析进一步证实了改性 SPIONs 和 PHBV 的成功结合。热重分析（TGA）表明，与 SPIONs 相比，PHBV 微球中掺入了 SPIONs。通过磁性测量也证明了这一点，因为检测到 PHBV/SPIONs 微球的这种磁性的更高值。通过用不同浓度的微球评估小鼠胚胎成纤维细胞（MEF），证明了磁性 PHBV 微球是无毒的。这些结果证实，所制备的磁性 PHBV 微球是生物医学应用的潜在候选材料。
