Faculty of Chemical and Food Technology, Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia.
Biotechnol J. 2019 Mar;14(3):e1800120. doi: 10.1002/biot.201800120. Epub 2018 May 17.
Early phase development of industrial immobilized biocatalysts has to address the selection of the best candidates from dozens of available carriers and binding methods. This work presents a simple selection method for the immobilization of industrial-grade Aspergillus oryzae β-galactosidase suitable for the production of galactooligosaccharides. Immobilization efficiency and yield of a variety of immobilized biocatalysts are evaluated using simple activity measurements and mathematical modeling of intraparticle kinetics and mass transfer. Activated carriers and some ion-exchange carriers provided highly active biocatalysts and are therefore selected for the investigation of GOS production in the batch reactor. Biocatalysts exhibited significant differences in the yield of galactooligosaccharides and productivity of GOS formation. Based on these process performance criteria, cost considerations are made that allow further selection of the best biocatalyst for future process optimization. The presented method of biocatalyst selection can be useful for other industrial enzymes applications.
工业固定化生物催化剂的早期开发必须解决从数十种可用载体和结合方法中选择最佳候选物的问题。本工作提出了一种简单的选择方法，用于固定化适合生产半乳糖低聚糖的工业级米曲霉β-半乳糖苷酶。使用简单的活性测量和颗粒内动力学和传质的数学建模来评估各种固定化生物催化剂的固定化效率和产率。活化载体和一些离子交换载体提供了高活性的生物催化剂，因此被选择用于在间歇式反应器中生产半乳糖低聚糖。生物催化剂在半乳糖低聚糖的产率和半乳糖低聚糖形成的生产力方面表现出显著差异。基于这些工艺性能标准，考虑成本因素，以便为未来的工艺优化进一步选择最佳的生物催化剂。所提出的生物催化剂选择方法可用于其他工业酶应用。
