He Ming Ming, Wang Xiu Feng, Gu Duan Yin, Shi Yu Liang, Han Hui Hui, Shi Qing Hua, Yang Feng Juan, Wei Min
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, China.
State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2017 Apr 18;28(4):1246-1254. doi: 10.13287/j.1001-9332.201704.015.
The solution culture method was used to study the effects of sodium nitroprusside (SNP) on plant growth, nutrient uptake and antioxidant enzyme activities of tomato seedlings under iron deficiency and NO stress. The results indicated that after 7 d of treatment, iron deficiency inhibited the tomato seedling growth, decreased chlorophyll (a and b) and carotenoid contents, and led to obvious chlorosis; iron deficiency also reduced the activity of SOD, POD and CAT, resulting in significant accumulation of MDA contents and higher electrolytic leakage; proline and soluble sugar contents were not significantly changed; contents of N, P, K, Ca, Mg and Fe in both leaves and roots of tomato seedlings were decreased under iron deficiency in varying degrees. The combined stress of NO stress and iron deficiency promoted the inhibition of plant growth, decreased chlorophyll (a and b), carotenoid contents and the activities of SOD, POD and CAT; electrolytic leakage MDA, proline and soluble sugar contents of N, P, Mg, Fe in both leaves and roots of tomato seedlings were much lower, but the contents of K and Ca were significantly increased. Compared to the treatment without SNP, adding 0.1 mmol·L SNP could alleviate the growth inhibition of stressed tomato seedlings. Adding 0.1 mmol·L SF (sodium ferrocyanide) also showed alleviating or promoting effect on some above indexes including the activity of SOD, POD and CAT to some degree, but the other physiological indexes were not significantly changed because iron was contained in SF.
采用溶液培养法研究了硝普钠（SNP）对缺铁和NO胁迫下番茄幼苗生长、养分吸收及抗氧化酶活性的影响。结果表明，处理7 d后，缺铁抑制了番茄幼苗生长，降低了叶绿素（a和b）及类胡萝卜素含量，导致明显的黄化现象；缺铁还降低了超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）的活性，导致丙二醛（MDA）含量显著积累和相对电导率升高；脯氨酸和可溶性糖含量无显著变化；缺铁条件下番茄幼苗叶片和根系中N、P、K、Ca、Mg和Fe含量均不同程度降低。NO胁迫和缺铁的复合胁迫加剧了对植株生长的抑制，降低了叶绿素（a和b）、类胡萝卜素含量以及SOD、POD和CAT的活性；番茄幼苗叶片和根系的相对电导率、MDA、脯氨酸和可溶性糖含量以及N、P、Mg、Fe含量均大幅降低，但K和Ca含量显著增加。与不添加SNP的处理相比，添加0.1 mmol·L SNP可缓解胁迫对番茄幼苗生长的抑制。添加0.1 mmol·L亚铁氰化钠（SF）对上述部分指标包括SOD、POD和CAT活性也有一定程度的缓解或促进作用，但由于SF中含铁，其他生理指标无显著变化。
