Bondan Eduardo Fernandes, Vieira Carolina Cardoso, Martins Maria de Fátima Monteiro, Kirsten Thiago Berti, Bernardi Maria Martha
Patologia Ambiental e Experimental, Universidade Paulista, São Paulo, SP, Brasil.
Arq Neuropsiquiatr. 2018 Apr;76(4):252-256. doi: 10.1590/0004-282x20180019.
Obesity is associated with a chronic and low-grade inflammatory response in the hypothalamus, where astrogliosis occurs with the upregulation of the astrocyte structural protein GFAP. As propentofylline (PPF) has inhibitory effects on astrocyte and microglial activation during inflammation, this study aimed to investigate if this xanthine derivative could decrease the astrocyte reaction induced by a hypercaloric diet (HD). Male Wistar rats were divided into four groups: NDS - rats receiving a normocaloric diet (ND) and daily saline solution; NDP - rats receiving ND and daily PPF (12.5 mg/kg/day, intraperitoneal route); HDS - rats receiving HD and saline solution, HDP - rats receiving HD and PPF. On the 21st day, rats were anesthetized, and perfused, and brains were collected for GFAP immunohistochemical study in the hypothalamus. Results showed that HD induced increased weight gain and hypothalamic astrogliosis. Propentofylline decreased the expression of GFAP in the HDP group, although it did not affect the weight gain induced by this diet.
肥胖与下丘脑的慢性低度炎症反应有关，在该区域会发生星形胶质细胞增生，同时星形胶质细胞结构蛋白胶质纤维酸性蛋白（GFAP）上调。由于丙戊茶碱（PPF）在炎症期间对星形胶质细胞和小胶质细胞的激活具有抑制作用，本研究旨在探讨这种黄嘌呤衍生物是否能减轻高热量饮食（HD）诱导的星形胶质细胞反应。雄性Wistar大鼠分为四组：NDS组——接受正常热量饮食（ND）并每日注射生理盐水的大鼠；NDP组——接受ND并每日注射PPF（12.5毫克/千克/天，腹腔注射）的大鼠；HDS组——接受HD并注射生理盐水的大鼠；HDP组——接受HD并注射PPF的大鼠。在第21天，将大鼠麻醉、灌注，然后收集大脑用于下丘脑GFAP免疫组织化学研究。结果显示，HD导致体重增加和下丘脑星形胶质细胞增生。丙戊茶碱降低了HDP组中GFAP的表达，尽管它并未影响这种饮食诱导的体重增加。
