Shrestha Rojeet, Miura Yusuke, Hirano Ken-Ichi, Chen Zhen, Okabe Hiroaki, Chiba Hitoshi, Hui Shu-Ping
Faculty of Health Sciences, Hokkaido University.
Laboratory of Cardiovascular Disease, Novel, Non-Invasive, and Nutritional Therapeutics (CNT), Graduate School of Medicine, Osaka University.
Anal Sci. 2018;34(5):575-582. doi: 10.2116/analsci.17P557.
Fatty acid (FA) profiling of milk has important applications in human health and nutrition. Conventional methods for the saponification and derivatization of FA are time-consuming and laborious. We aimed to develop a simple, rapid, and economical method for the determination of FA in milk. We applied a beneficial approach of microwave-assisted saponification (MAS) of milk fats and microwave-assisted derivatization (MAD) of FA to its hydrazides, integrated with HPLC-based analysis. The optimal conditions for MAS and MAD were determined. Microwave irradiation significantly reduced the sample preparation time from 80 min in the conventional method to less than 3 min. We used three internal standards for the measurement of short-, medium- and long-chain FA. The proposed method showed satisfactory analytical sensitivity, recovery and reproducibility. There was a significant correlation in the milk FA concentrations between the proposed and conventional methods. Being quick, economic, and convenient, the proposed method for the milk FA measurement can be substitute for the convention method.
牛奶脂肪酸（FA）分析在人类健康和营养方面具有重要应用。传统的脂肪酸皂化和衍生化方法既耗时又费力。我们旨在开发一种简单、快速且经济的牛奶脂肪酸测定方法。我们采用了一种有益的方法，即对乳脂肪进行微波辅助皂化（MAS），并将脂肪酸微波辅助衍生化为其酰肼（MAD），同时结合基于高效液相色谱（HPLC）的分析方法。确定了MAS和MAD的最佳条件。微波辐射显著缩短了样品制备时间，从传统方法的80分钟减少到不到3分钟。我们使用了三种内标来测量短链、中链和长链脂肪酸。所提出的方法具有令人满意的分析灵敏度、回收率和重现性。所提出的方法与传统方法测得的牛奶脂肪酸浓度之间存在显著相关性。所提出的牛奶脂肪酸测量方法快速、经济且方便，可替代传统方法。
