Belchior Diana C V, Duarte Iola F, Freire Mara G
CICECO-Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Aveiro, Portugal.
Adv Biochem Eng Biotechnol. 2019;168:1-29. doi: 10.1007/10_2018_66.
Bioseparation processes are a relevant part of modern biotechnology, particularly regarding the development of efficient and biocompatible methods for the separation and purification of added-value biologically active compounds. In this field, ionic liquids (ILs) have been proposed, either in liquid-liquid extractions, in which non-water miscible ILs or aqueous biphasic systems (ABS) formed by ILs can be used, or in solid-liquid extractions, in which they are covalently attached to create supported IL phases (SILPs). Aprotic ILs possess unique properties, such as non-volatility and designability, which are valuable in their use in bioseparation processes. In this chapter, we summarize and discuss bioseparation processes based on ILs, including both liquid-liquid and solid-liquid extractions, applied to amino acids and proteins. The most recent and remarkable advances in this area are emphasized, and improvements brought by the use of ILs properly discussed. New insights and envisaged directions with IL-based bioseparation processes are suggested. Graphical Abstract.
生物分离过程是现代生物技术的一个重要组成部分，特别是在开发用于分离和纯化具有附加值的生物活性化合物的高效且生物相容的方法方面。在该领域，离子液体（ILs）已被提出用于液 - 液萃取，其中可以使用与水不混溶的离子液体或由离子液体形成的双水相系统（ABS），或者用于固 - 液萃取，其中它们通过共价连接形成负载型离子液体相（SILPs）。非质子离子液体具有诸如不挥发性和可设计性等独特性质，这些性质在其用于生物分离过程中很有价值。在本章中，我们总结并讨论基于离子液体的生物分离过程，包括应用于氨基酸和蛋白质的液 - 液萃取和固 - 液萃取。强调了该领域最新且显著的进展，并适当讨论了使用离子液体带来的改进。还提出了基于离子液体的生物分离过程的新见解和设想方向。图形摘要。
