Marusa Borgonio-Cuadra Veronica, Miranda-Duarte Antonio, Rojas-Toledo Xochitl, Garcia-Hernandez Normand, Alfredo Sierra-Ramirez Jose, Cardenas-Garcia Maura, Elena Hernandez-Caballero Marta
Instituto Nacional de Rehabilitación, Laboratorio de Genética, Calzada México Xochimilco 289, Delegación Tlalpan, Arenal de Guadalupe, 14389, México City, México.
J BUON. 2018 Mar-Apr;23(2):361-365.
Aberrant methylation of CpG islands in the promoter is a hallmark of cancer, leading to transcriptional silencing of tumor suppressor genes. The aim of this work was to evaluate the promoter methylation status of the DACT2 gene in breast cancer (BC) tissue and to analyze its possible effect on tumor type or grade.
CpG island from the DACT2 promoter in region -240 to -14 from transcriptional start site (TSS) were obtained. Through the use of sodium bisulfite DNA conversion analysis, followed by detection with MSP (methylation specific PCR), we analyzed 79 BC and 15 adjacent healthy samples.
T he c ases a nalyzed w ere i n s tage I ( 2.5%), I I (38%), or III (59.5%). The most frequent tumor type was invasive ductal carcinoma (71.4%). Methylation analysis comparing tumor tissues with adjacent non-cancerous tissues showed statistical significance. Methylation was observed in 32.9% (26/79) of the samples; no methylation was found in adjacent healthy tissue. DACT2 methylation was associated with tumor stage I-II (p=0.03) and stage III (p=0.004).
An association was found of DACT2 promoter methylation with advanced tumor stages. This gene has been suggested as a potential biomarker, however, more investigation is required to validate this function.
启动子区域CpG岛的异常甲基化是癌症的一个标志，会导致肿瘤抑制基因的转录沉默。本研究旨在评估乳腺癌（BC）组织中DACT2基因启动子的甲基化状态，并分析其对肿瘤类型或分级的可能影响。
获取转录起始位点（TSS）-240至-14区域内DACT2启动子的CpG岛。通过亚硫酸氢钠DNA转化分析，随后用甲基化特异性PCR（MSP）进行检测，我们分析了79例乳腺癌样本和15例相邻健康样本。
所分析的病例处于I期（2.5%）、II期（38%）或III期（59.5%）。最常见的肿瘤类型是浸润性导管癌（71.4%）。肿瘤组织与相邻非癌组织的甲基化分析显示具有统计学意义。在32.9%（26/79）的样本中观察到甲基化；在相邻健康组织中未发现甲基化。DACT2甲基化与肿瘤I-II期（p = 0.03）和III期（p = 0.004）相关。
发现DACT2启动子甲基化与肿瘤晚期有关。该基因已被认为是一种潜在的生物标志物，然而，需要更多的研究来验证这一功能。
