Deguchi K, Shirakawa S
2nd Department of Internal Medicine, Faculty of Medicine, Mie University, Japan.
Thromb Res Suppl. 1988;8:65-72. doi: 10.1016/0049-3848(88)90155-7.
Platelets were found to provide a surface for activation of plasminogen by the tissue-type plasminogen activator (t-PA) at an optimum concentration and to potentiate the generation of plasmin by the amidolytic method, fibrin lysis time and fibrin plate method. The effect of platelets on amidolytic activity on S-2251 was due to the potentiating effect of plasminogen activation by t-PA, because it was observed only in the presence of both plasminogen and t-PA. Plasmin generation was also evidenced in the SDS-PAGE profile of the supernatant from a mixture containing t-PA and plasminogen with platelets. These findings suggests that the potentiating activity of platelets on plasminogen activation by t-PA in circulation is one of the causes of fibrinogenolysis during fibrinolytic therapy with a high dose of t-PA. Platelets from patients with various diseases showed different potentiating activity on plasminogen activation by t-PA. The assay of this ability of platelets may be a new tool for evaluating their role in the blood fibrinolytic process.
研究发现，血小板能够在组织型纤溶酶原激活剂（t-PA）的最佳浓度下为纤溶酶原的激活提供一个表面，并通过酰胺水解法、纤维蛋白溶解时间和纤维蛋白平板法增强纤溶酶的生成。血小板对S-2251酰胺水解活性的影响是由于t-PA对纤溶酶原激活的增强作用，因为这种影响仅在同时存在纤溶酶原和t-PA的情况下才会观察到。在含有t-PA、纤溶酶原和血小板的混合物上清液的SDS-PAGE图谱中也证实了纤溶酶的生成。这些发现表明，血小板在循环中对t-PA激活纤溶酶原的增强活性是高剂量t-PA溶栓治疗期间纤维蛋白原溶解的原因之一。患有各种疾病的患者的血小板对t-PA激活纤溶酶原表现出不同的增强活性。检测血小板的这种能力可能是评估其在血液纤溶过程中作用的一种新工具。
