Gin T E, Puchot M L, Cook A K
Veterinary Medical Teaching Hospital, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 408 Raymond Stotzer Pkwy, College Station, TX 77845, USA.
Veterinary Medical Teaching Hospital, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 408 Raymond Stotzer Pkwy, College Station, TX 77845, USA.
Domest Anim Endocrinol. 2018 Jul;64:66-69. doi: 10.1016/j.domaniend.2018.03.002. Epub 2018 Mar 19.
Baseline cortisol concentrations are routinely used to screen dogs for hypoadrenocorticism (HOC); this diagnosis must then be confirmed with an ACTH stimulation test. A baseline cortisol concentration less than 55 nmol/L (2 μg/dL) is highly sensitive for HOC but lacks specificity, with a false positive rate >20%. Many dogs with nonadrenal disease are therefore subjected to unnecessary additional testing. It was hypothesized that exposure to an unpleasant auditory stimulus before sample collection would improve the specificity of baseline cortisol measurements in dogs with nonadrenal disease by triggering cortisol production. Twenty-eight healthy client-owned dogs were included in the study, with a median age of 4 yr (range 2-9 yr) and a median weight of 20 kg (range 10-27 kg). Dogs were ineligible for inclusion if they had received short- or long-acting glucocorticoids within the previous 30 and 90 d, respectively. Dogs were randomly assigned to group 1 (control; no noise; n = 7), group 2 (brief noise: n = 10), or group 3 (long noise: n = 11). Each dog and owner were directed to a secluded area for approximately 15 min. Group 1 sat in relative quiet, exposed only to the background sounds of a veterinary hospital. Group 2 were exposed to the sound of a wet-dry vacuum in an adjacent hallway during the first 3 min of this period. Group 3 were exposed to random bursts of wet-dry vacuum noise during this period. At the end of the test interval, each dog was escorted to an adjacent examination room for blood collection. Samples were processed within 15 min; serum was frozen at -80°C before measurement of cortisol concentrations. Median serum cortisol concentrations and the proportion of dogs with results <55 nmol/L were similar for the 3 groups. The study hypothesis that exposure to the noise of a wet-dry vacuum cleaner would consistently drive baseline serum cortisol concentrations above 55 nmol/L in dogs with apparently normal adrenal function was therefore rejected.
基础皮质醇浓度通常用于筛查犬的肾上腺皮质功能减退症（HOC）；然后必须通过促肾上腺皮质激素（ACTH）刺激试验来确诊。基础皮质醇浓度低于55 nmol/L（2 μg/dL）对HOC具有高度敏感性，但缺乏特异性，假阳性率>20%。因此，许多患有非肾上腺疾病的犬接受了不必要的额外检查。据推测，在样本采集前暴露于不愉快的听觉刺激会通过触发皮质醇分泌来提高患有非肾上腺疾病犬的基础皮质醇测量的特异性。28只健康的客户拥有的犬被纳入该研究，中位年龄为4岁（范围2 - 9岁），中位体重为20 kg（范围10 - 27 kg）。如果犬在之前的30天和90天内分别接受过短效或长效糖皮质激素治疗，则不符合纳入标准。犬被随机分配到第1组（对照组；无噪音；n = 7）、第2组（短暂噪音组：n = 10）或第3组（长时间噪音组：n = 11）。每只犬和主人被引导到一个僻静的区域约15分钟。第1组相对安静地坐着，仅暴露于兽医医院的背景声音中。第2组在此期间的前3分钟暴露于相邻走廊干湿两用吸尘器的声音中。第3组在此期间暴露于干湿两用吸尘器的随机突发噪音中。在测试间隔结束时，每只犬被护送到相邻的检查室进行采血。样本在15分钟内进行处理；血清在测量皮质醇浓度前于-80°C冷冻。3组犬的血清皮质醇浓度中位数以及结果<55 nmol/L的犬的比例相似。因此，关于暴露于干湿两用吸尘器噪音会使肾上腺功能明显正常的犬的基础血清皮质醇浓度持续高于55 nmol/L的研究假设被否定。
