Mullinax Rebecca, Johns Sarah E, Rhodes Denise, Zhang Vivian, McKinney Nancy, Felts Katherine A, Carstens Carsten P, Sheffield Peter
Genomics R&D, Agilent Technologies Inc., La Jolla, CA, USA.
Methods Mol Biol. 2018;1772:457-468. doi: 10.1007/978-1-4939-7795-6_27.
Vector construction and gene cloning are ubiquitous techniques essential to all fields of biological and medical research. They are the first steps in many endeavors leading to expressing proteins to understand gene function and regulation. However, they can often be rate-limiting, particularly in multi-gene studies, due to the time and effort required to assemble gene constructs and to identify the optimal constructs for protein expression.The SureVector system was developed to address this by enabling the rapid and reliable assembly of multiple DNA modules into a recombinant plasmid containing a gene-of-interest (GOI). It harnesses the power of synthetic biology to combine DNA modules from standard parts into a customized vector that expresses proteins in bacterial, mammalian, or yeast cells. The key advantages of the innovative SureVector system include rapid custom vector generation, enhanced flexibility to assemble new vectors quickly as experimental requirements change, and the reliable and precise assembly of fully interchangeable standard DNA modules that retain their functionality. The SureVector system is the only next-generation plasmid assembly technology to guarantee assembly of multiple functional DNA modules.
载体构建和基因克隆是生物和医学研究所有领域普遍使用的重要技术。它们是许多旨在表达蛋白质以了解基因功能和调控的研究工作的第一步。然而，由于组装基因构建体以及鉴定用于蛋白质表达的最佳构建体所需的时间和精力，它们常常可能成为限速步骤，尤其是在多基因研究中。SureVector系统的开发就是为了解决这一问题，它能够将多个DNA模块快速、可靠地组装到一个含有目的基因（GOI）的重组质粒中。它利用合成生物学的力量，将来自标准部件的DNA模块组合成一个定制载体，该载体可在细菌、哺乳动物或酵母细胞中表达蛋白质。创新的SureVector系统的主要优点包括快速生成定制载体、随着实验要求的变化增强快速组装新载体的灵活性，以及可靠、精确地组装完全可互换且保留其功能的标准DNA模块。SureVector系统是唯一能保证多个功能性DNA模块组装的下一代质粒组装技术。
