赖氨酰氧化酶与 ADAMTS 蛋白之间的相互作用提示两种细胞外基质家族之间存在新的串扰。
Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families.
机构信息
The Rappaport Faculty of Medicine and Research Institute, Technion - Israel Institute of Technology, Haifa 31096, Israel.
Department of Biomedical Engineering, Cleveland Clinic Lerner Research Institute, Cleveland, OH, 44120, USA.
出版信息
Matrix Biol. 2019 Jan;75-76:114-125. doi: 10.1016/j.matbio.2018.05.003. Epub 2018 May 17.
Abstract
The extracellular matrix (ECM) regulates numerous cellular events in addition to providing structural integrity. Among several protein and enzyme families implicated in functions of the ECM, the lysyl oxidases and ADAMTS proteins are known to participate in microfibril and elastic fiber formation as well as ECM-associated signaling. A yeast two-hybrid screen to identify lysyl oxidase (LOX) binding proteins identified ADAMTSL4 as a potential interactor. We demonstrate here that several members of the LOX and ADAMTS families interact with one another. Upon investigating the interaction between LOX and ADAMTSL2 we found that the absence or inhibition of Lox affected ADAMTSL2 molecular forms and reduced its tissue levels. Thus, ADAMTSL2 stability and inter-molecular complexes may depend on the activity of lysyl oxidases.
摘要
细胞外基质 (ECM) 除了提供结构完整性外，还调节许多细胞事件。在几种参与 ECM 功能的蛋白质和酶家族中，赖氨酰氧化酶和 ADAMTS 蛋白已知参与微纤维和弹性纤维的形成以及 ECM 相关的信号转导。酵母双杂交筛选以鉴定赖氨酰氧化酶 (LOX) 结合蛋白，鉴定 ADAMTSL4 为潜在的相互作用蛋白。我们在这里证明 LOX 和 ADAMTS 家族的几个成员相互作用。在研究 LOX 和 ADAMTSL2 之间的相互作用时，我们发现 Lox 的缺失或抑制会影响 ADAMTSL2 的分子形式并降低其组织水平。因此，ADAMTSL2 的稳定性和分子间复合物可能依赖于赖氨酰氧化酶的活性。
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