Olayaki Luqman Aribidesi, Alagbonsi Isiaka Abdullateef, Abdulrahim Amin Halimat, Adeyemi Wale Johnson, Bakare Muftiat, Omeiza Noah
1 Department of Physiology, College of Health Sciences, University of Ilorin, Ilorin, Kwara, Nigeria.
2 Department of Physiology, School of Medicine and Pharmacy, University of Rwanda College of Medicine and Health Sciences, Huye, Butare, Republic of Rwanda.
Toxicol Ind Health. 2018 Sep;34(9):596-608. doi: 10.1177/0748233718773508. Epub 2018 May 15.
We investigated the effects of melatonin on sperm parameters and some biochemical markers in lead-exposed male Wistar rats. Lead (50 mg/kg bw/day) and/or melatonin (4 mg/kg or 10 mg/kg bw/day) was administered for 4 weeks, while 2-week lead exposure was preceded by or followed by 2-week treatment with both doses of melatonin in other groups. Lead reduced glutathione, catalase, adjusted testes weight, semen parameters but did not change malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase, and total antioxidant capacity. Though independent of prolactin, lead-induced gonadotoxicity was both centrally and peripherally mediated, as it reduced gonadotropin-releasing hormone and testosterone levels, while gonadotropin levels did not change significantly probably due to negative feedback by elevated estradiol. However, pre-, simultaneous, or posttreatment of lead-exposed rats with melatonin reduced MDA, SOD, and estradiol but dose-dependently increased other parameters. Conclusively, lead causes male gonadotoxicity through oxidative stress and endocrine mechanisms, and these could be dose-dependently prevented and ameliorated by melatonin.
我们研究了褪黑素对铅暴露雄性Wistar大鼠精子参数和一些生化标志物的影响。给予铅（50毫克/千克体重/天）和/或褪黑素（4毫克/千克或10毫克/千克体重/天），持续4周，而在其他组中，在2周铅暴露之前或之后用两种剂量的褪黑素进行2周治疗。铅降低了谷胱甘肽、过氧化氢酶、调整后的睾丸重量、精液参数，但未改变丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶和总抗氧化能力。虽然与催乳素无关，但铅诱导的性腺毒性是由中枢和外周介导的，因为它降低了促性腺激素释放激素和睾酮水平，而促性腺激素水平可能由于雌二醇升高的负反馈而没有显著变化。然而，用褪黑素对铅暴露大鼠进行预处理、同时处理或后处理可降低MDA、SOD和雌二醇，但剂量依赖性地增加了其他参数。总之，铅通过氧化应激和内分泌机制导致雄性性腺毒性，而褪黑素可以剂量依赖性地预防和改善这些毒性。
