Shingu T, Karanian J W, Kim H Y, Yergey J A, Salem N
Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, Bethesda, MD 20892.
Adv Alcohol Subst Abuse. 1988;7(3-4):235-40. doi: 10.1300/J251v07n03_31.
It is known that alcohol exposure leads to a decline in brain docosahexaenoate (22:6w3). We hypothesized that alcohol could stimulate the metabolism of this polyunsaturated fatty acid to bioactive products. Several oxidized products of 22:6w3 were indeed observed when rat brain homogenate was incubated with 14C22:6w3 in vitro. A similar group of metabolites was formed in vivo from 14C-22:6w3 injected into the lateral ventricle. These metabolites were characterized by thermospray- and GC/MS as well as by the synthesis of standards using purified enzymes. Platelet lipoxygenase also proved useful in identifying one of the brain metabolites and served as a source of enzyme for preparative studies. Their physiological effects on smooth muscle tone and platelet aggregation will be presented.
已知酒精暴露会导致大脑二十二碳六烯酸（22:6w3）含量下降。我们推测酒精可能会刺激这种多不饱和脂肪酸代谢为生物活性产物。当大鼠脑匀浆在体外与14C22:6w3一起孵育时，确实观察到了22:6w3的几种氧化产物。从注入侧脑室的14C-22:6w3在体内也形成了类似的一组代谢物。这些代谢物通过热喷雾和气相色谱/质谱以及使用纯化酶合成标准品进行了表征。血小板脂氧合酶在鉴定一种脑代谢物方面也证明是有用的，并作为制备研究的酶来源。将介绍它们对平滑肌张力和血小板聚集的生理作用。
