粗糙脉孢菌新的 stopper 突变体 ER-3 线粒体 DNA 的分子特征分析。
Molecular characterization of the mitochondrial DNA of a new stopper mutant ER-3 of Neurospora crassa.
作者信息
Almasan A, Mishra N C
机构信息
Department of Biology, University of South Carolina, Columbia 29208.
出版信息
Genetics. 1988 Dec;120(4):935-45. doi: 10.1093/genetics/120.4.935.
Abstract
An ethidium bromide-induced stopper mutant of Neurospora crassa is characterized at the molecular level. The mutant has two populations of mitochondrial DNA: a defective predominant mutant molecule and a basal level of the wild-type molecule. The aberrant DNA resulted after a 25-kbp deletion from the wild-type mitochondrial chromosome, which included major genes such as cytb, co1 and oli2. The deletion endpoints are located in the second intron of the ND5 gene, and in a sequence 250 nucleotides upstream of the co2 gene. The recombination has taken place between two nine nucleotide repeats CCCCGCCCC, one of which is close to a PstI palindrome at its 5' end. Thus the mutant ER-3 differs from all the other stopper mutants described previously in the extent and location of the deletions in the mtDNA.
摘要
对由溴化乙锭诱导产生的粗糙脉孢菌终止子突变体进行了分子水平的表征。该突变体有两种线粒体DNA群体：一种是有缺陷的占主导地位的突变分子，另一种是野生型分子的基础水平。异常DNA是在野生型线粒体染色体发生25 kbp的缺失后产生的，其中包括细胞色素b、细胞色素氧化酶亚基I和寡霉素敏感相关蛋白2等主要基因。缺失端点位于ND5基因的第二个内含子以及细胞色素氧化酶亚基II基因上游250个核苷酸的序列中。重组发生在两个九核苷酸重复序列CCCCGCCCC之间，其中一个在其5'端靠近PstI回文序列。因此，突变体ER-3在mtDNA缺失的程度和位置上与先前描述的所有其他终止子突变体不同。
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