Yin S, Heckman J, RajBhandary U L
Cell. 1981 Nov;26(3 Pt 1):325-32. doi: 10.1016/0092-8674(81)90201-4.
In sequencing a 2200 bp region of the Neurospora crassa mitochondrial DNA encoding the 3' end of the large rRNA gene and a cluster of six tRNA genes, we have found that the tRNA genes are flanked by highly conserved GC-rich palindromic DNA sequences. An 18 bp long core sequence, 5'-CC CTGCAG TA CTGCAG GG-3', containing two closely spaced Pst I sites, is common to all these palindromic sequences. Each of the eight Pst I sites mapped in the 2200 bp region consists of two closely spaced Pst I sites; thus this 2200 bp long segment actually contains 16 Pst I sites. Between 5-10% of the N. crassa DNA may consist of these GC-rich palindromic sequences that include the 18 base long core sequence. The same core sequence is present within both the 5' and 3' side of the intervening sequence of the large rRNA gene, close to, but not at, the intron-exon boundaries. We discuss probable roles for these sequences in N. crassa mitochondrial function, including their role as signals either in the synthesis or processing (or both) of RNA in the mitochondria.
在对粗糙脉孢菌线粒体DNA的一个2200 bp区域进行测序时，该区域编码大rRNA基因的3'端和一组六个tRNA基因，我们发现tRNA基因两侧是高度保守的富含GC的回文DNA序列。一个18 bp长的核心序列，5'-CC CTGCAG TA CTGCAG GG-3'，包含两个紧密间隔的Pst I位点，是所有这些回文序列所共有的。在2200 bp区域中定位的八个Pst I位点中的每一个都由两个紧密间隔的Pst I位点组成；因此，这个2200 bp长的片段实际上包含16个Pst I位点。粗糙脉孢菌DNA的5%-10%可能由这些富含GC的回文序列组成，这些序列包括18个碱基长的核心序列。相同的核心序列存在于大rRNA基因间隔序列的5'和3'侧，靠近但不在内含子-外显子边界处。我们讨论了这些序列在粗糙脉孢菌线粒体功能中的可能作用，包括它们作为线粒体中RNA合成或加工（或两者）信号的作用。
