Leite J P, Collard J F, D'Halluin J C
Laboratoire de Virologie Moléculaire, INSERM, U. 233, Lille, France.
Gene. 1988 Sep 15;69(1):111-20. doi: 10.1016/0378-1119(88)90383-6.
HeLa cells were co-transfected with recombinant plasmids carrying adenovirus (Ad)2 or Ad3 E1a promoters fused to the chloramphenicol acetyl transferase gene (cat), and a plasmid encoding the Ad3 E1a promoter. Whereas no stimulating effect was observed on the Ad3 E1a promoter, the Ad2 promoter was inhibited. To determine which of the E1a gene products of Ad3 was responsible for the repressive effect, plasmids were constructed in which only the 13S or 12S mRNA product of Ad3 was expressed. Both the 12S and 13S mRNA products of Ad3 E1a were found to depress the transcription from the Ad2 E1a promoter. Each Ad3 E1a gene product was able to stimulate transcription from the Ad5 E2a early promoter in a manner similar to that of the Ad2 E1a gene products. In the case of the Ad5 E3 promoter, neither of the Ad3 E1a gene products stimulated transcription, but an inhibition was observed. These results suggest that both mRNA products of the Ad3 E1a region inhibit transcription at the TATA box transcription complex.
将携带与氯霉素乙酰转移酶基因（cat）融合的腺病毒（Ad）2或Ad3 E1a启动子的重组质粒，与编码Ad3 E1a启动子的质粒共转染HeLa细胞。虽然未观察到对Ad3 E1a启动子有刺激作用，但Ad2启动子受到抑制。为了确定Ad3的E1a基因产物中哪一种对这种抑制作用负责，构建了仅表达Ad3的13S或12S mRNA产物的质粒。发现Ad3 E1a的12S和13S mRNA产物均能抑制Ad2 E1a启动子的转录。每个Ad3 E1a基因产物都能够以类似于Ad2 E1a基因产物的方式刺激Ad5 E2a早期启动子的转录。对于Ad5 E3启动子，Ad3 E1a基因产物均未刺激转录，但观察到有抑制作用。这些结果表明，Ad3 E1a区域的两种mRNA产物均在TATA盒转录复合体处抑制转录。
