Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, South Korea.
Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Ansan Hospital, Ansan, South Korea.
Int J Clin Oncol. 2018 Oct;23(5):851-859. doi: 10.1007/s10147-018-1294-3. Epub 2018 May 14.
Recently, the genetic alterations associated with tumor progression and impaired host immunity against transformed cells draw increased attention. Here, we characterized the differential gene expression patterns and protein expression in tumor-free lymph node from recurrent and non-recurrent tumors to identify independent prognostic markers for oral squamous cell carcinoma (OSCC).
A cDNA microarray analysis was performed to identify the differentially expressed genes in regional tumor-free lymph nodes from OSCC patients with and without recurrence. Then, the protein expression of the selected genes was analyzed by immunohistochemistry in 60 OSCC patients to determine their association with survival.
Widespread down-regulation of genes involved in antigen processing and recognition in lymph nodes was a distinctive feature. In univariate Kaplan-Meier analysis, lower expression of CD40L and CD80 in tumor-free lymph nodes was significantly correlated with poorer survival. In multivariate Cox regression analysis, CD40L was identified as an independent prognostic marker of disease-free survival.
Our data indicate that impaired host immunity (decreased CD40L expression) along with the TNM staging might be an important factor determining the prognosis of OSCC.
最近，与肿瘤进展和宿主对转化细胞免疫功能受损相关的遗传改变引起了越来越多的关注。在这里，我们对复发性和非复发性肿瘤无肿瘤淋巴结中的差异基因表达模式和蛋白表达进行了特征分析，以确定口腔鳞状细胞癌（OSCC）的独立预后标志物。
我们通过 cDNA 微阵列分析鉴定了复发性和非复发性 OSCC 患者无肿瘤淋巴结中的差异表达基因。然后，通过免疫组织化学分析 60 例 OSCC 患者中选定基因的蛋白表达情况，以确定它们与生存的相关性。
广泛下调与抗原加工和识别相关的基因是淋巴结的一个显著特征。在单变量 Kaplan-Meier 分析中，无肿瘤淋巴结中 CD40L 和 CD80 的低表达与较差的生存显著相关。在多变量 Cox 回归分析中，CD40L 被确定为无病生存的独立预后标志物。
我们的数据表明，宿主免疫功能受损（CD40L 表达降低）以及 TNM 分期可能是决定 OSCC 预后的一个重要因素。
